Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues

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Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues. / Alanazi, Asma; Munir, Hafsa; Alassiri, Mohammed; Ward, Lewis S C; McGettrick, Helen; Nash, Gerard.

In: Biorheology, Vol. 56, No. 1, 10.05.2019, p. 15-30.

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Alanazi, Asma ; Munir, Hafsa ; Alassiri, Mohammed ; Ward, Lewis S C ; McGettrick, Helen ; Nash, Gerard. / Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues. In: Biorheology. 2019 ; Vol. 56, No. 1. pp. 15-30.

Bibtex

@article{df6927a97df344a985a10964f634bace,
title = "Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues",
abstract = "Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood. OBJECTIVE: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue. METHODS: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 um pore filters coated with the same proteins was analysed. RESULTS: The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSCBMMSCTBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSCTBMSC=UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC. CONCLUSIONS: MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.",
keywords = "Mesenchymal stem cells, bone marrow, cell adhesion, cell migration, collagen, fibronectin, umbilical cord",
author = "Asma Alanazi and Hafsa Munir and Mohammed Alassiri and Ward, {Lewis S C} and Helen McGettrick and Gerard Nash",
year = "2019",
month = may
day = "10",
doi = "10.3233/BIR-180185",
language = "English",
volume = "56",
pages = "15--30",
journal = "Biorheology",
issn = "0006-355X",
publisher = "IOS Press",
number = "1",

}

RIS

TY - JOUR

T1 - Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues

AU - Alanazi, Asma

AU - Munir, Hafsa

AU - Alassiri, Mohammed

AU - Ward, Lewis S C

AU - McGettrick, Helen

AU - Nash, Gerard

PY - 2019/5/10

Y1 - 2019/5/10

N2 - Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood. OBJECTIVE: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue. METHODS: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 um pore filters coated with the same proteins was analysed. RESULTS: The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSCBMMSCTBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSCTBMSC=UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC. CONCLUSIONS: MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.

AB - Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood. OBJECTIVE: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue. METHODS: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 um pore filters coated with the same proteins was analysed. RESULTS: The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSCBMMSCTBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSCTBMSC=UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC. CONCLUSIONS: MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.

KW - Mesenchymal stem cells

KW - bone marrow

KW - cell adhesion

KW - cell migration

KW - collagen

KW - fibronectin

KW - umbilical cord

UR - http://www.scopus.com/inward/record.url?scp=85065643463&partnerID=8YFLogxK

U2 - 10.3233/BIR-180185

DO - 10.3233/BIR-180185

M3 - Article

VL - 56

SP - 15

EP - 30

JO - Biorheology

JF - Biorheology

SN - 0006-355X

IS - 1

ER -