Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells

Research output: Contribution to journalArticlepeer-review

Standard

Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells. / Papadopoulos, Angelos; Chalmantzi, Varvara; Mikhaylichenko, Olga; Stellas, Dimitris; Hyvonen, Marko; Kanhere, Aditi; Heath, John; Cunningham, Debbie; Fotsis, Theodore; Murphy, Carol.

In: Stem Cell Research, Vol. 50, 102133, 01.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Papadopoulos, Angelos ; Chalmantzi, Varvara ; Mikhaylichenko, Olga ; Stellas, Dimitris ; Hyvonen, Marko ; Kanhere, Aditi ; Heath, John ; Cunningham, Debbie ; Fotsis, Theodore ; Murphy, Carol. / Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells. In: Stem Cell Research. 2021 ; Vol. 50.

Bibtex

@article{7754bb5ceae343e888ceff414d4589aa,
title = "Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells",
abstract = "Human embryonic stem cells (hESCs) are an invaluable tool in the fields of embryology and regenerative medicine. Activin A and BMP4 are well-characterised growth factors implicated in pluripotency and differentiation. In the current study, hESCs are cultured in a modified version of mTeSR1, where low concentrations of Activin A substitute for TGFβ. This culture system is further used to investigate the changes induced by BMP4 on hESCs by employing a combination of transcriptomic and phosphoproteomic approaches. Results indicate that in a pluripotent state, hESCs maintain WNT signaling under negative regulation by expressing pathway inhibitors. Initial stages of differentiation are characterized by upregulation of WNT pathway ligands, TGFβ pathway inhibitors which have been shown in Xenopus to expand the BMP signaling range essential for embryonic patterning, and mesendodermal transcripts. Moreover, BMP4 enhances the phosphorylation of proteins associated with migration and transcriptional regulation. Results further indicate the vital regulatory role of Activin A and BMP4 in crucial fate decisions in hESCs.",
keywords = "Activin A, BMP4, Human embryonic stem cells, Phosphoproteomics, Transcriptomics",
author = "Angelos Papadopoulos and Varvara Chalmantzi and Olga Mikhaylichenko and Dimitris Stellas and Marko Hyvonen and Aditi Kanhere and John Heath and Debbie Cunningham and Theodore Fotsis and Carol Murphy",
note = "Funding Information: This work was supported by funding from the School of Biosciences, University of Birmingham.",
year = "2021",
month = jan,
doi = "10.1016/j.scr.2020.102133",
language = "English",
volume = "50",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells

AU - Papadopoulos, Angelos

AU - Chalmantzi, Varvara

AU - Mikhaylichenko, Olga

AU - Stellas, Dimitris

AU - Hyvonen, Marko

AU - Kanhere, Aditi

AU - Heath, John

AU - Cunningham, Debbie

AU - Fotsis, Theodore

AU - Murphy, Carol

N1 - Funding Information: This work was supported by funding from the School of Biosciences, University of Birmingham.

PY - 2021/1

Y1 - 2021/1

N2 - Human embryonic stem cells (hESCs) are an invaluable tool in the fields of embryology and regenerative medicine. Activin A and BMP4 are well-characterised growth factors implicated in pluripotency and differentiation. In the current study, hESCs are cultured in a modified version of mTeSR1, where low concentrations of Activin A substitute for TGFβ. This culture system is further used to investigate the changes induced by BMP4 on hESCs by employing a combination of transcriptomic and phosphoproteomic approaches. Results indicate that in a pluripotent state, hESCs maintain WNT signaling under negative regulation by expressing pathway inhibitors. Initial stages of differentiation are characterized by upregulation of WNT pathway ligands, TGFβ pathway inhibitors which have been shown in Xenopus to expand the BMP signaling range essential for embryonic patterning, and mesendodermal transcripts. Moreover, BMP4 enhances the phosphorylation of proteins associated with migration and transcriptional regulation. Results further indicate the vital regulatory role of Activin A and BMP4 in crucial fate decisions in hESCs.

AB - Human embryonic stem cells (hESCs) are an invaluable tool in the fields of embryology and regenerative medicine. Activin A and BMP4 are well-characterised growth factors implicated in pluripotency and differentiation. In the current study, hESCs are cultured in a modified version of mTeSR1, where low concentrations of Activin A substitute for TGFβ. This culture system is further used to investigate the changes induced by BMP4 on hESCs by employing a combination of transcriptomic and phosphoproteomic approaches. Results indicate that in a pluripotent state, hESCs maintain WNT signaling under negative regulation by expressing pathway inhibitors. Initial stages of differentiation are characterized by upregulation of WNT pathway ligands, TGFβ pathway inhibitors which have been shown in Xenopus to expand the BMP signaling range essential for embryonic patterning, and mesendodermal transcripts. Moreover, BMP4 enhances the phosphorylation of proteins associated with migration and transcriptional regulation. Results further indicate the vital regulatory role of Activin A and BMP4 in crucial fate decisions in hESCs.

KW - Activin A

KW - BMP4

KW - Human embryonic stem cells

KW - Phosphoproteomics

KW - Transcriptomics

UR - http://www.scopus.com/inward/record.url?scp=85098489928&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2020.102133

DO - 10.1016/j.scr.2020.102133

M3 - Article

VL - 50

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 102133

ER -