TY - CHAP
T1 - Collection and Preparation of Clinical Samples for Metabolomics.
AU - Dunn, Warwick
AU - Chetwynd, Andrew
AU - Rodriguez Blanco, Giovanny
PY - 2017/2/1
Y1 - 2017/2/1
N2 - A wide range of biofluids (urine, serum, plasma, saliva, etc.) as well as cellular and tissue samples can be collected and investigated in clinical metabolomic studies. The choice of sample is dependent on the clinical question being investigated with biofluids typically studied to identify biomarkers, whereas tissues and primary/immortalised cells are typically studied to investigate mechanisms associated with pathophysiological processes. Methods applied to collect samples, quench metabolism and extract samples differ between sample types from simple collect, dilute and analyse methods for urine to complex washing, quenching and biphasic extraction methods for tissues. The range of sample collection and extraction methods are discussed with sample-specific considerations highlighted. Finally, methods for imaging of cells and tissues and for in vivo metabolomic analysis will also be introduced.
AB - A wide range of biofluids (urine, serum, plasma, saliva, etc.) as well as cellular and tissue samples can be collected and investigated in clinical metabolomic studies. The choice of sample is dependent on the clinical question being investigated with biofluids typically studied to identify biomarkers, whereas tissues and primary/immortalised cells are typically studied to investigate mechanisms associated with pathophysiological processes. Methods applied to collect samples, quench metabolism and extract samples differ between sample types from simple collect, dilute and analyse methods for urine to complex washing, quenching and biphasic extraction methods for tissues. The range of sample collection and extraction methods are discussed with sample-specific considerations highlighted. Finally, methods for imaging of cells and tissues and for in vivo metabolomic analysis will also be introduced.
U2 - 10.1007/978-3-319-47656-8_2
DO - 10.1007/978-3-319-47656-8_2
M3 - Chapter
SN - 9783319476551
T3 - Advances in Experimental Medicine and Biology
SP - 19
BT - Metabolomics: From Fundamentals to Clinical Applications
PB - Springer
ER -