Coexpression of flt-1, flt-4 and KDR in freshly isolated and cultured human endothelial cells

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

Vascular endothelial cell growth factors (VEGF) are key modulators of endothelial cell growth and function. The class III receptor tyrosine kinases KDR and Flt-1 are high affinity receptors for VEGF, while Flt-4 is a receptor for the recently identified VEGF-C. We have examined the expression of flt-1, flt-4 and KDR in human microvascular and large vessel endothelial cells and in a variety of other cell types in vitro. Endothelial cells proliferated and exhibited increased procoagulant activity in response to VEGF. Flt-1, flt-4 and KDR were detected in both freshly isolated endothelial cells, and in sparse and confluent endothelial cell cultures by RT-PCR. Attempts to modulate receptor expression by culturing cells at reduced oxygen tensions (2%) did not induce consistent changes in flt-1, flt-4 or KDR expression. Incubation with tumor-conditioned medium or co-culture of endothelial cells with a range of breast and small cell lung carcinoma cell lines did not reproducibly alter receptor mRNA expression. However, flt-1, flt-4 and KDR transcript levels were enhanced following treatment with tetradecanoylphorbol acetate.

Details

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number3
Publication statusPublished - 25 Apr 1996

Keywords

  • Base Sequence, Cells, Cultured, DNA Primers, Endothelium, Vascular, Humans, Molecular Sequence Data, Receptor Protein-Tyrosine Kinases, Receptors, Growth Factor