Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins

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Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins. / Batah, Jameel; Kobeissy, Hussein; Bui Pham, Phuong Trang; Denève-Larrazet, Cécile; Kuehne, Sarah; Collignon, Anne; Janoir-Jouveshomme, Claire; Marvaud, Jean-Christophe; Kansau, Imad.

In: Scientific Reports, Vol. 7, No. 1, 12.06.2017, p. 3256.

Research output: Contribution to journalArticle

Harvard

Batah, J, Kobeissy, H, Bui Pham, PT, Denève-Larrazet, C, Kuehne, S, Collignon, A, Janoir-Jouveshomme, C, Marvaud, J-C & Kansau, I 2017, 'Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins', Scientific Reports, vol. 7, no. 1, pp. 3256. https://doi.org/10.1038/s41598-017-03621-z

APA

Batah, J., Kobeissy, H., Bui Pham, P. T., Denève-Larrazet, C., Kuehne, S., Collignon, A., Janoir-Jouveshomme, C., Marvaud, J-C., & Kansau, I. (2017). Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins. Scientific Reports, 7(1), 3256. https://doi.org/10.1038/s41598-017-03621-z

Vancouver

Author

Batah, Jameel ; Kobeissy, Hussein ; Bui Pham, Phuong Trang ; Denève-Larrazet, Cécile ; Kuehne, Sarah ; Collignon, Anne ; Janoir-Jouveshomme, Claire ; Marvaud, Jean-Christophe ; Kansau, Imad. / Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins. In: Scientific Reports. 2017 ; Vol. 7, No. 1. pp. 3256.

Bibtex

@article{d5674186abf3493faf8dbad550c874b2,
title = "Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins",
abstract = "Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the gut, but other bacterial factors are needed for the bacteria to adhere and colonize the intestinal epithelium. The C. difficile flagella, which confer motility and chemotaxis for successful intestinal colonization, could play an additional role in bacterial pathogenesis by contributing to the inflammatory response of the host and mucosal injury. Indeed, by activating the TLR5, flagella can elicit activation of the MAPK and NF-κB cascades of cell signaling, leading to the secretion of pro-inflammatory cytokines. In the current study, we demonstrate, by using an animal model of CDI, a synergic effect of flagella and toxins in eliciting an inflammatory mucosal response. In this model, the absence of flagella dramatically decreases the degree of mucosal inflammation in mice and the sole presence of toxins without flagella was not enough to elicit epithelial lesions. These results highlight the important role of C. difficile flagella in eliciting mucosal lesions as long as the toxins exert their action on the epithelium.",
author = "Jameel Batah and Hussein Kobeissy and {Bui Pham}, {Phuong Trang} and C{\'e}cile Den{\`e}ve-Larrazet and Sarah Kuehne and Anne Collignon and Claire Janoir-Jouveshomme and Jean-Christophe Marvaud and Imad Kansau",
year = "2017",
month = jun,
day = "12",
doi = "10.1038/s41598-017-03621-z",
language = "English",
volume = "7",
pages = "3256",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins

AU - Batah, Jameel

AU - Kobeissy, Hussein

AU - Bui Pham, Phuong Trang

AU - Denève-Larrazet, Cécile

AU - Kuehne, Sarah

AU - Collignon, Anne

AU - Janoir-Jouveshomme, Claire

AU - Marvaud, Jean-Christophe

AU - Kansau, Imad

PY - 2017/6/12

Y1 - 2017/6/12

N2 - Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the gut, but other bacterial factors are needed for the bacteria to adhere and colonize the intestinal epithelium. The C. difficile flagella, which confer motility and chemotaxis for successful intestinal colonization, could play an additional role in bacterial pathogenesis by contributing to the inflammatory response of the host and mucosal injury. Indeed, by activating the TLR5, flagella can elicit activation of the MAPK and NF-κB cascades of cell signaling, leading to the secretion of pro-inflammatory cytokines. In the current study, we demonstrate, by using an animal model of CDI, a synergic effect of flagella and toxins in eliciting an inflammatory mucosal response. In this model, the absence of flagella dramatically decreases the degree of mucosal inflammation in mice and the sole presence of toxins without flagella was not enough to elicit epithelial lesions. These results highlight the important role of C. difficile flagella in eliciting mucosal lesions as long as the toxins exert their action on the epithelium.

AB - Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the gut, but other bacterial factors are needed for the bacteria to adhere and colonize the intestinal epithelium. The C. difficile flagella, which confer motility and chemotaxis for successful intestinal colonization, could play an additional role in bacterial pathogenesis by contributing to the inflammatory response of the host and mucosal injury. Indeed, by activating the TLR5, flagella can elicit activation of the MAPK and NF-κB cascades of cell signaling, leading to the secretion of pro-inflammatory cytokines. In the current study, we demonstrate, by using an animal model of CDI, a synergic effect of flagella and toxins in eliciting an inflammatory mucosal response. In this model, the absence of flagella dramatically decreases the degree of mucosal inflammation in mice and the sole presence of toxins without flagella was not enough to elicit epithelial lesions. These results highlight the important role of C. difficile flagella in eliciting mucosal lesions as long as the toxins exert their action on the epithelium.

U2 - 10.1038/s41598-017-03621-z

DO - 10.1038/s41598-017-03621-z

M3 - Article

C2 - 28607468

VL - 7

SP - 3256

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -