Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

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Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter. / Hewett, P W; Daft, E L; Murray, J C.

In: Biochemical and Biophysical Research Communications, Vol. 252, No. 3, 27.11.1998, p. 546-51.

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@article{d28c7fe574a8470388292b0b055c1537,
title = "Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter",
abstract = "The Tie-2 receptor plays a key role in vascular development, although little is known about the factors controlling its expression. Here we report the first cloning and characterisation of the 5' regulatory region of human tie-2. Multiple transcription start sites were identified between -414 and -265 bp upstream of the start codon using 5' RACE, fluorescent primer extension, and RNase protection assays. The human tie-2 promoter contains several transcription factor-binding sequences including ets, SP-1, AP-1, and GATA-1, but there are no canonical TATA or CCAAT initiation sequences proximal to the transcription start sites. Human tie-2 reporter constructs demonstrated approximately 10-fold greater activity in endothelial cells compared with fibroblasts. In endothelial cells the tie-2 promoter exhibited 5 and 16% of the activity of human tie-1 (830 bp) and KDR (1.1 kb) promoters, respectively. This promoter will be a useful tool for studying factors that regulate tie-2 expression and targeting the vasculature.",
keywords = "Animals, Base Sequence, Cattle, Cells, Cultured, Cloning, Molecular, Endothelium, Vascular, Humans, Molecular Sequence Data, Promoter Regions, Genetic, Receptor Protein-Tyrosine Kinases, Receptor, TIE-2, Transcription, Genetic",
author = "Hewett, {P W} and Daft, {E L} and Murray, {J C}",
note = "Copyright 1998 Academic Press.",
year = "1998",
month = nov,
day = "27",
doi = "10.1006/bbrc.1998.9690",
language = "English",
volume = "252",
pages = "546--51",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

AU - Hewett, P W

AU - Daft, E L

AU - Murray, J C

N1 - Copyright 1998 Academic Press.

PY - 1998/11/27

Y1 - 1998/11/27

N2 - The Tie-2 receptor plays a key role in vascular development, although little is known about the factors controlling its expression. Here we report the first cloning and characterisation of the 5' regulatory region of human tie-2. Multiple transcription start sites were identified between -414 and -265 bp upstream of the start codon using 5' RACE, fluorescent primer extension, and RNase protection assays. The human tie-2 promoter contains several transcription factor-binding sequences including ets, SP-1, AP-1, and GATA-1, but there are no canonical TATA or CCAAT initiation sequences proximal to the transcription start sites. Human tie-2 reporter constructs demonstrated approximately 10-fold greater activity in endothelial cells compared with fibroblasts. In endothelial cells the tie-2 promoter exhibited 5 and 16% of the activity of human tie-1 (830 bp) and KDR (1.1 kb) promoters, respectively. This promoter will be a useful tool for studying factors that regulate tie-2 expression and targeting the vasculature.

AB - The Tie-2 receptor plays a key role in vascular development, although little is known about the factors controlling its expression. Here we report the first cloning and characterisation of the 5' regulatory region of human tie-2. Multiple transcription start sites were identified between -414 and -265 bp upstream of the start codon using 5' RACE, fluorescent primer extension, and RNase protection assays. The human tie-2 promoter contains several transcription factor-binding sequences including ets, SP-1, AP-1, and GATA-1, but there are no canonical TATA or CCAAT initiation sequences proximal to the transcription start sites. Human tie-2 reporter constructs demonstrated approximately 10-fold greater activity in endothelial cells compared with fibroblasts. In endothelial cells the tie-2 promoter exhibited 5 and 16% of the activity of human tie-1 (830 bp) and KDR (1.1 kb) promoters, respectively. This promoter will be a useful tool for studying factors that regulate tie-2 expression and targeting the vasculature.

KW - Animals

KW - Base Sequence

KW - Cattle

KW - Cells, Cultured

KW - Cloning, Molecular

KW - Endothelium, Vascular

KW - Humans

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Receptor Protein-Tyrosine Kinases

KW - Receptor, TIE-2

KW - Transcription, Genetic

U2 - 10.1006/bbrc.1998.9690

DO - 10.1006/bbrc.1998.9690

M3 - Article

C2 - 9837743

VL - 252

SP - 546

EP - 551

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -