Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

The Tie-2 receptor plays a key role in vascular development, although little is known about the factors controlling its expression. Here we report the first cloning and characterisation of the 5' regulatory region of human tie-2. Multiple transcription start sites were identified between -414 and -265 bp upstream of the start codon using 5' RACE, fluorescent primer extension, and RNase protection assays. The human tie-2 promoter contains several transcription factor-binding sequences including ets, SP-1, AP-1, and GATA-1, but there are no canonical TATA or CCAAT initiation sequences proximal to the transcription start sites. Human tie-2 reporter constructs demonstrated approximately 10-fold greater activity in endothelial cells compared with fibroblasts. In endothelial cells the tie-2 promoter exhibited 5 and 16% of the activity of human tie-1 (830 bp) and KDR (1.1 kb) promoters, respectively. This promoter will be a useful tool for studying factors that regulate tie-2 expression and targeting the vasculature.

Details

Original languageEnglish
Pages (from-to)546-51
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number3
Publication statusPublished - 27 Nov 1998

Keywords

  • Animals, Base Sequence, Cattle, Cells, Cultured, Cloning, Molecular, Endothelium, Vascular, Humans, Molecular Sequence Data, Promoter Regions, Genetic, Receptor Protein-Tyrosine Kinases, Receptor, TIE-2, Transcription, Genetic