Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

A Molina; F Biemar; F Müller; A Iyengar; P Prunet; N Maclean; J A Martial; M Muller

Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

A Molina, F Biemar, F Müller, A Iyengar, P Prunet, N Maclean, J A Martial, M Muller

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.

Original language	English
Pages (from-to)	5-10
Number of pages	6
Journal	FEBS Letters
Volume	474
Issue number	1
Publication status	Published - 26 May 2000

Keywords

Amino Acid Sequence
Animals
Base Sequence
Carps
Cloning, Molecular
DNA
Gene Expression
HSP70 Heat-Shock Proteins
Hot Temperature
Molecular Sequence Data
Promoter Regions, Genetic
RNA, Messenger
Regulatory Sequences, Nucleic Acid
Reverse Transcriptase Polymerase Chain Reaction
TATA Box
Tilapia
Transfection
Zebrafish

Cite this

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title = "Cloning and expression analysis of an inducible HSP70 gene from tilapia fish",

abstract = "We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Carps, Cloning, Molecular, DNA, Gene Expression, HSP70 Heat-Shock Proteins, Hot Temperature, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger, Regulatory Sequences, Nucleic Acid, Reverse Transcriptase Polymerase Chain Reaction, TATA Box, Tilapia, Transfection, Zebrafish",

author = "A Molina and F Biemar and F M{\"u}ller and A Iyengar and P Prunet and N Maclean and Martial, {J A} and M Muller",

year = "2000",

month = may,

day = "26",

language = "English",

volume = "474",

pages = "5--10",

journal = "FEBS Letters",

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}

TY - JOUR

T1 - Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

AU - Molina, A

AU - Biemar, F

AU - Müller, F

AU - Iyengar, A

AU - Prunet, P

AU - Maclean, N

AU - Martial, J A

AU - Muller, M

PY - 2000/5/26

Y1 - 2000/5/26

N2 - We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.

AB - We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Carps

KW - Cloning, Molecular

KW - DNA

KW - Gene Expression

KW - HSP70 Heat-Shock Proteins

KW - Hot Temperature

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Regulatory Sequences, Nucleic Acid

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - TATA Box

KW - Tilapia

KW - Transfection

KW - Zebrafish

M3 - Article

C2 - 10828441

SN - 0014-5793

VL - 474

SP - 5

EP - 10

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

Abstract

Keywords

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