Class-switch recombination occurs infrequently in germinal centers

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Class-switch recombination occurs infrequently in germinal centers. / Roco, Jonathan; Mesin, Luka; Binder, Sebastian C; Nefzger, Christian; Gonzalez-Figueroa, Paula; Canete, Pablo F; Ellyard, Julia; Shen, Qian; Robert, Philippe A; Cappello, Jean; Vohra, Harpreet; Zhang, Yang; Nowasad, Carla R; Schiepers, Arien; Corcoran, Lynn M; Toellner, Kai; Polo, Jose M; Meyer-Hermann, Michael; Victora, Gabriel D; Vinuesa, Carola G.

In: Immunity, Vol. 51, No. 2, e7, 20.08.2019, p. 337-350.

Research output: Contribution to journalArticle

Harvard

Roco, J, Mesin, L, Binder, SC, Nefzger, C, Gonzalez-Figueroa, P, Canete, PF, Ellyard, J, Shen, Q, Robert, PA, Cappello, J, Vohra, H, Zhang, Y, Nowasad, CR, Schiepers, A, Corcoran, LM, Toellner, K, Polo, JM, Meyer-Hermann, M, Victora, GD & Vinuesa, CG 2019, 'Class-switch recombination occurs infrequently in germinal centers', Immunity, vol. 51, no. 2, e7, pp. 337-350. https://doi.org/10.1016/j.immuni.2019.07.001

APA

Roco, J., Mesin, L., Binder, S. C., Nefzger, C., Gonzalez-Figueroa, P., Canete, P. F., Ellyard, J., Shen, Q., Robert, P. A., Cappello, J., Vohra, H., Zhang, Y., Nowasad, C. R., Schiepers, A., Corcoran, L. M., Toellner, K., Polo, J. M., Meyer-Hermann, M., Victora, G. D., & Vinuesa, C. G. (2019). Class-switch recombination occurs infrequently in germinal centers. Immunity, 51(2), 337-350. [e7]. https://doi.org/10.1016/j.immuni.2019.07.001

Vancouver

Roco J, Mesin L, Binder SC, Nefzger C, Gonzalez-Figueroa P, Canete PF et al. Class-switch recombination occurs infrequently in germinal centers. Immunity. 2019 Aug 20;51(2):337-350. e7. https://doi.org/10.1016/j.immuni.2019.07.001

Author

Roco, Jonathan ; Mesin, Luka ; Binder, Sebastian C ; Nefzger, Christian ; Gonzalez-Figueroa, Paula ; Canete, Pablo F ; Ellyard, Julia ; Shen, Qian ; Robert, Philippe A ; Cappello, Jean ; Vohra, Harpreet ; Zhang, Yang ; Nowasad, Carla R ; Schiepers, Arien ; Corcoran, Lynn M ; Toellner, Kai ; Polo, Jose M ; Meyer-Hermann, Michael ; Victora, Gabriel D ; Vinuesa, Carola G. / Class-switch recombination occurs infrequently in germinal centers. In: Immunity. 2019 ; Vol. 51, No. 2. pp. 337-350.

Bibtex

@article{686115e6906a45f4be187c00ab529679,
title = "Class-switch recombination occurs infrequently in germinal centers",
abstract = "Class-switch recombination (CSR) is a DNA recombination process that replaces the immunoglobulin (Ig) constant region for the isotype that can best protect against the pathogen. Dysregulation of CSR can cause self-reactive BCRs and B cell lymphomas; understanding the timing and location of CSR is therefore important. Although CSR commences upon T cell priming, it is generally considered a hallmark of germinal centers (GCs). Here, we have used multiple approaches to show that CSR is triggered prior to differentiation into GC B cells or plasmablasts and is greatly diminished in GCs. Despite finding a small percentage of GC B cells expressing germline transcripts, phylogenetic trees of GC BCRs from secondary lymphoid organs revealed that the vast majority of CSR events occurred prior to the onset of somatic hypermutation. As such, we have demonstrated the existence of IgM-dominated GCs, which are unlikely to occur under the assumption of ongoing switching.",
author = "Jonathan Roco and Luka Mesin and Binder, {Sebastian C} and Christian Nefzger and Paula Gonzalez-Figueroa and Canete, {Pablo F} and Julia Ellyard and Qian Shen and Robert, {Philippe A} and Jean Cappello and Harpreet Vohra and Yang Zhang and Nowasad, {Carla R} and Arien Schiepers and Corcoran, {Lynn M} and Kai Toellner and Polo, {Jose M} and Michael Meyer-Hermann and Victora, {Gabriel D} and Vinuesa, {Carola G}",
year = "2019",
month = aug
day = "20",
doi = "10.1016/j.immuni.2019.07.001",
language = "English",
volume = "51",
pages = "337--350",
journal = "Immunity",
issn = "1074-7613",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Class-switch recombination occurs infrequently in germinal centers

AU - Roco, Jonathan

AU - Mesin, Luka

AU - Binder, Sebastian C

AU - Nefzger, Christian

AU - Gonzalez-Figueroa, Paula

AU - Canete, Pablo F

AU - Ellyard, Julia

AU - Shen, Qian

AU - Robert, Philippe A

AU - Cappello, Jean

AU - Vohra, Harpreet

AU - Zhang, Yang

AU - Nowasad, Carla R

AU - Schiepers, Arien

AU - Corcoran, Lynn M

AU - Toellner, Kai

AU - Polo, Jose M

AU - Meyer-Hermann, Michael

AU - Victora, Gabriel D

AU - Vinuesa, Carola G

PY - 2019/8/20

Y1 - 2019/8/20

N2 - Class-switch recombination (CSR) is a DNA recombination process that replaces the immunoglobulin (Ig) constant region for the isotype that can best protect against the pathogen. Dysregulation of CSR can cause self-reactive BCRs and B cell lymphomas; understanding the timing and location of CSR is therefore important. Although CSR commences upon T cell priming, it is generally considered a hallmark of germinal centers (GCs). Here, we have used multiple approaches to show that CSR is triggered prior to differentiation into GC B cells or plasmablasts and is greatly diminished in GCs. Despite finding a small percentage of GC B cells expressing germline transcripts, phylogenetic trees of GC BCRs from secondary lymphoid organs revealed that the vast majority of CSR events occurred prior to the onset of somatic hypermutation. As such, we have demonstrated the existence of IgM-dominated GCs, which are unlikely to occur under the assumption of ongoing switching.

AB - Class-switch recombination (CSR) is a DNA recombination process that replaces the immunoglobulin (Ig) constant region for the isotype that can best protect against the pathogen. Dysregulation of CSR can cause self-reactive BCRs and B cell lymphomas; understanding the timing and location of CSR is therefore important. Although CSR commences upon T cell priming, it is generally considered a hallmark of germinal centers (GCs). Here, we have used multiple approaches to show that CSR is triggered prior to differentiation into GC B cells or plasmablasts and is greatly diminished in GCs. Despite finding a small percentage of GC B cells expressing germline transcripts, phylogenetic trees of GC BCRs from secondary lymphoid organs revealed that the vast majority of CSR events occurred prior to the onset of somatic hypermutation. As such, we have demonstrated the existence of IgM-dominated GCs, which are unlikely to occur under the assumption of ongoing switching.

UR - http://www.scopus.com/inward/record.url?scp=85070659620&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2019.07.001

DO - 10.1016/j.immuni.2019.07.001

M3 - Article

VL - 51

SP - 337

EP - 350

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 2

M1 - e7

ER -