cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution

Research output: Contribution to journalArticle

Standard

cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution. / Goettgens, B; Ferreira, R; Sanchez, MJ; Ishibashi, S; Li, J; Spensberger, D; Lefevre, P; Ottersbach, K; Chapman, M; Kinston, S; Knezevic, K; Hoogenkamp, Maarten; Follows, GA; Bonifer, Constanze; Amaya, E; Green, AR.

In: Molecular and Cellular Biology, Vol. 30, No. 24, 01.12.2010, p. 5741-5751.

Research output: Contribution to journalArticle

Harvard

Goettgens, B, Ferreira, R, Sanchez, MJ, Ishibashi, S, Li, J, Spensberger, D, Lefevre, P, Ottersbach, K, Chapman, M, Kinston, S, Knezevic, K, Hoogenkamp, M, Follows, GA, Bonifer, C, Amaya, E & Green, AR 2010, 'cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution', Molecular and Cellular Biology, vol. 30, no. 24, pp. 5741-5751. https://doi.org/10.1128/MCB.00870-10

APA

Goettgens, B., Ferreira, R., Sanchez, MJ., Ishibashi, S., Li, J., Spensberger, D., Lefevre, P., Ottersbach, K., Chapman, M., Kinston, S., Knezevic, K., Hoogenkamp, M., Follows, GA., Bonifer, C., Amaya, E., & Green, AR. (2010). cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution. Molecular and Cellular Biology, 30(24), 5741-5751. https://doi.org/10.1128/MCB.00870-10

Vancouver

Goettgens B, Ferreira R, Sanchez MJ, Ishibashi S, Li J, Spensberger D et al. cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution. Molecular and Cellular Biology. 2010 Dec 1;30(24):5741-5751. https://doi.org/10.1128/MCB.00870-10

Author

Goettgens, B ; Ferreira, R ; Sanchez, MJ ; Ishibashi, S ; Li, J ; Spensberger, D ; Lefevre, P ; Ottersbach, K ; Chapman, M ; Kinston, S ; Knezevic, K ; Hoogenkamp, Maarten ; Follows, GA ; Bonifer, Constanze ; Amaya, E ; Green, AR. / cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution. In: Molecular and Cellular Biology. 2010 ; Vol. 30, No. 24. pp. 5741-5751.

Bibtex

@article{32a0a84b12814b408b6fea1c2f5589f2,
title = "cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution",
abstract = "Development progresses through a sequence of cellular identities which are determined by the activities of networks of transcription factor genes. Alterations in cis-regulatory elements of these genes play a major role in evolutionary change, but little is known about the mechanisms responsible for maintaining conserved patterns of gene expression. We have studied the evolution of cis-regulatory mechanisms controlling the SCL gene, which encodes a key transcriptional regulator of blood, vasculature, and brain development and exhibits conserved function and pattern of expression throughout vertebrate evolution. SCL cis-regulatory elements are conserved between frog and chicken but accrued alterations at an accelerated rate between 310 and 200 million years ago, with subsequent fixation of a new cis-regulatory pattern at the beginning of the mammalian radiation. As a consequence, orthologous elements shared by mammals and lower vertebrates exhibit functional differences and binding site turnover between widely separated cis-regulatory modules. However, the net effect of these alterations is constancy of overall regulatory inputs and of expression pattern. Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution.",
keywords = "Vertebrate Evolution, SCL Locus",
author = "B Goettgens and R Ferreira and MJ Sanchez and S Ishibashi and J Li and D Spensberger and P Lefevre and K Ottersbach and M Chapman and S Kinston and K Knezevic and Maarten Hoogenkamp and GA Follows and Constanze Bonifer and E Amaya and AR Green",
year = "2010",
month = dec,
day = "1",
doi = "10.1128/MCB.00870-10",
language = "English",
volume = "30",
pages = "5741--5751",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "24",

}

RIS

TY - JOUR

T1 - cis-Regulatory Remodeling of the SCL Locus during Vertebrate Evolution

AU - Goettgens, B

AU - Ferreira, R

AU - Sanchez, MJ

AU - Ishibashi, S

AU - Li, J

AU - Spensberger, D

AU - Lefevre, P

AU - Ottersbach, K

AU - Chapman, M

AU - Kinston, S

AU - Knezevic, K

AU - Hoogenkamp, Maarten

AU - Follows, GA

AU - Bonifer, Constanze

AU - Amaya, E

AU - Green, AR

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Development progresses through a sequence of cellular identities which are determined by the activities of networks of transcription factor genes. Alterations in cis-regulatory elements of these genes play a major role in evolutionary change, but little is known about the mechanisms responsible for maintaining conserved patterns of gene expression. We have studied the evolution of cis-regulatory mechanisms controlling the SCL gene, which encodes a key transcriptional regulator of blood, vasculature, and brain development and exhibits conserved function and pattern of expression throughout vertebrate evolution. SCL cis-regulatory elements are conserved between frog and chicken but accrued alterations at an accelerated rate between 310 and 200 million years ago, with subsequent fixation of a new cis-regulatory pattern at the beginning of the mammalian radiation. As a consequence, orthologous elements shared by mammals and lower vertebrates exhibit functional differences and binding site turnover between widely separated cis-regulatory modules. However, the net effect of these alterations is constancy of overall regulatory inputs and of expression pattern. Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution.

AB - Development progresses through a sequence of cellular identities which are determined by the activities of networks of transcription factor genes. Alterations in cis-regulatory elements of these genes play a major role in evolutionary change, but little is known about the mechanisms responsible for maintaining conserved patterns of gene expression. We have studied the evolution of cis-regulatory mechanisms controlling the SCL gene, which encodes a key transcriptional regulator of blood, vasculature, and brain development and exhibits conserved function and pattern of expression throughout vertebrate evolution. SCL cis-regulatory elements are conserved between frog and chicken but accrued alterations at an accelerated rate between 310 and 200 million years ago, with subsequent fixation of a new cis-regulatory pattern at the beginning of the mammalian radiation. As a consequence, orthologous elements shared by mammals and lower vertebrates exhibit functional differences and binding site turnover between widely separated cis-regulatory modules. However, the net effect of these alterations is constancy of overall regulatory inputs and of expression pattern. Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution.

KW - Vertebrate Evolution

KW - SCL Locus

U2 - 10.1128/MCB.00870-10

DO - 10.1128/MCB.00870-10

M3 - Article

C2 - 20956563

VL - 30

SP - 5741

EP - 5751

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 24

ER -