Circulating soluble adhesion molecules in systemic vasculitis

Research output: Contribution to journalArticlepeer-review

Authors

  • A. A. Pall
  • M. Drayson
  • N. T. Richards
  • J. Michael
  • D. Adu

Colleges, School and Institutes

External organisations

  • Queen Elizabeth Hospital Birmingham
  • Department of Immunology

Abstract

The plasma levels of soluble intercellular adhesion molecule-1 (sICAM-1), E-selectin (sE-selectin), and vascular cell adhesion molecule-1 (sVCAM-1), might reflect endothelial activation and injury and would therefore be useful markers of disease activity in vasculitis. To investigate this we measured the levels of sICAM-1, sE-selectin, and sVCAM-1 by two-site ELISAs in the plasma of patients with (a) active vasculitis (n = 16), (b) vasculitis in remission (n = 15), (c) chronic renal failure (CRF) (n = 10), and (d) normal healthy controls (n = 10). Plasma sICAM-1 levels were significantly higher in patients with active vasculitis, 323 ng/ml (193–607) compared with patients with inactive vasculitis, 199 ng/ml (131–297); P = 0.0006 and healthy controls, 188 ng/ml (138–259); P =0.0002. Plasma sE-selectin levels were also significantly higher in the patients with active vasculitis, 45 ng/ml (15–65) compared with patients with inactive vasculitis, 25 ng7sol;ml (15–55); P=0.027 but not when compared with healthy controls, 35 ng/ml (20–55); P=0.16. There was no difference in plasma sVCAM-1 levels between patients with active vasculitis, OD 0.56 (0.45–0.85) and inactive disease, OD 0.58 (0.47–0.79) (P=0.12) or with healthy controls OD 0.49 (0.42–0.68) (P=0.48). There were no significant differences between the plasma levels of any of the soluble adhe sion molecules between patients with active vasculitis and patients with chronic failure. In patients with a vasculitis there was a significant correlation between sICAM-1 and plasma C-reactive protein (CRP) (r=0.60, P=<0.01) and plasma von Willebrand factor (vWF) (r=0.42, P<0.05). Likewise there was a cor relation between sE-selectin and CRP (r=0.45, P<0.02) but not with vWF. There was a significant correlation between sICAM-1 and sE-selectin (r=0.38, P<0.05), but not between sICAM-1 and sVCAM-1 or sE- selectin and sVCAM-1. No correlation was found between sVCAM-1 levels and CRP and vWF concentrations or between the levels of any of the soluble adhesion molecules and serum creatinine. Plasma levels of sICAM-i and of sE-selectin but not of sVCAM-1 reflect disease activity in vasculitis and may be markers of endothelial and or tissue injury in these disorders.

Details

Original languageEnglish
Pages (from-to)770-774
Number of pages5
JournalNephrology Dialysis Transplantation
Volume9
Issue number7
Publication statusPublished - 1 Jan 1994

Keywords

  • Adhesion molecules, Endothelium, Vasculitis

ASJC Scopus subject areas