Chromatographic analysis of the trinitrophenyl derivatives of insulin

G R Wallace; A McLeod; B M Chain

Chromatographic analysis of the trinitrophenyl derivatives of insulin

G R Wallace, A McLeod, B M Chain

Inflammation and Ageing

Research output: Contribution to journal › Article › peer-review

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Abstract

The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.

Original language	English
Pages (from-to)	239-46
Number of pages	8
Journal	J. Chromatogr
Volume	427
Issue number	2
Publication status	Published - 3 Jun 1988

Keywords

Chromatography, High Pressure Liquid
Dansyl Compounds
Hydrolysis
Indicators and Reagents
Insulin
Molecular Conformation
Spectrophotometry, Ultraviolet

Cite this

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title = "Chromatographic analysis of the trinitrophenyl derivatives of insulin",

abstract = "The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.",

keywords = "Chromatography, High Pressure Liquid, Dansyl Compounds, Hydrolysis, Indicators and Reagents, Insulin, Molecular Conformation, Spectrophotometry, Ultraviolet",

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AU - McLeod, A

AU - Chain, B M

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N2 - The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.

AB - The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.

KW - Chromatography, High Pressure Liquid

KW - Dansyl Compounds

KW - Hydrolysis

KW - Indicators and Reagents

KW - Insulin

KW - Molecular Conformation

KW - Spectrophotometry, Ultraviolet

M3 - Article

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VL - 427

SP - 239

EP - 246

JO - J. Chromatogr

JF - J. Chromatogr

IS - 2

ER -

Chromatographic analysis of the trinitrophenyl derivatives of insulin

Abstract

Keywords

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