Chromatographic analysis of the trinitrophenyl derivatives of insulin

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Colleges, School and Institutes


The insulin molecule was derivatised by reaction with trinitrobenzenesulphonic acid (TNBS), which is known to react predominantly with free primary amino groups. The products of the reaction were analysed by reversed-phase chromatography and by further derivatisation with dansyl chloride. Under the conditions of these experiments, TNBS was found to react preferentially with glycine at position A1. This finding is discussed in terms of the tertiary structure and immunogenicity of this derivative.


Original languageEnglish
Pages (from-to)239-46
Number of pages8
JournalJ. Chromatogr
Issue number2
Publication statusPublished - 3 Jun 1988


  • Chromatography, High Pressure Liquid, Dansyl Compounds, Hydrolysis, Indicators and Reagents, Insulin, Molecular Conformation, Spectrophotometry, Ultraviolet