TY - JOUR
T1 - Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site
AU - Pattanapipitpaisal, Pranee
AU - Brown, Nigel
AU - Macaskie, Lynne
PY - 2001/10/1
Y1 - 2001/10/1
N2 - A Gram-positive, hexavalent chromium [chromate: Cr(Vl)]-tolerant bacterium. isolated from tannery waste from Pakistan, was identified as a Microbacterium sp. by 16S rRNA gene sequence homology. The strain (designated as MP30) reduced toxic Cr(VI) only under anaerobic conditions at the expense of acetate as the electron donor. The bacterium was able to grow aerobically in L-broth supplemented with 15 mM CrO42- but then did not reduce Cr(VI). At a concentration of 2.4X10(9) cells/ml, 100 muM sodium chromate was reduced within 30 h; however, the maximum specific reduction rate was obtained at lower initial cell concentrations.
AB - A Gram-positive, hexavalent chromium [chromate: Cr(Vl)]-tolerant bacterium. isolated from tannery waste from Pakistan, was identified as a Microbacterium sp. by 16S rRNA gene sequence homology. The strain (designated as MP30) reduced toxic Cr(VI) only under anaerobic conditions at the expense of acetate as the electron donor. The bacterium was able to grow aerobically in L-broth supplemented with 15 mM CrO42- but then did not reduce Cr(VI). At a concentration of 2.4X10(9) cells/ml, 100 muM sodium chromate was reduced within 30 h; however, the maximum specific reduction rate was obtained at lower initial cell concentrations.
UR - http://www.scopus.com/inward/record.url?scp=0034789671&partnerID=8YFLogxK
U2 - 10.1007/s002530100758
DO - 10.1007/s002530100758
M3 - Article
SN - 1432-0614
SN - 1432-0614
VL - 57
SP - 257
EP - 261
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1-2
ER -