ChREBP regulates Pdx-1 and other glucose-sensitive genes in pancreatic β-cells

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ChREBP regulates Pdx-1 and other glucose-sensitive genes in pancreatic β-cells. / da Silva Xavier, Gabriela; Sun, Gao; Qian, Qingwen; Rutter, Guy A; Leclerc, Isabelle.

In: Biochemical and Biophysical Research Communications, Vol. 402, No. 2, 12.11.2010, p. 252-257.

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da Silva Xavier, Gabriela ; Sun, Gao ; Qian, Qingwen ; Rutter, Guy A ; Leclerc, Isabelle. / ChREBP regulates Pdx-1 and other glucose-sensitive genes in pancreatic β-cells. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 402, No. 2. pp. 252-257.

Bibtex

@article{0eb922e8adcc4175b4ba3146dd1d5236,
title = "ChREBP regulates Pdx-1 and other glucose-sensitive genes in pancreatic β-cells",
abstract = "Carbohydrate responsive element-binding protein (ChREBP) is a transcription factor whose expression and activity are increased in pancreatic β-cells maintained at elevated glucose concentrations. We show here that ChREBP inactivation in clonal pancreatic MIN6 β-cells results in an increase in Pdx-1 expression at low glucose and to a small, but significant, increase in Ins2, GcK and MafA gene expression at high glucose concentrations. Conversely, adenovirus-mediated over-expression of ChREBP in mouse pancreatic islets results in decreases in Pdx-1, MafA, Ins1, Ins2 and GcK mRNA levels. These data suggest that strategies to reduce ChREBP activity might protect against β-cell dysfunction in type 2 diabetes.",
keywords = "Animals, Base Sequence, Cell Line, Tumor, Gene Expression Regulation, Gene Silencing, Glucose/metabolism, Homeodomain Proteins/genetics, Insulin-Secreting Cells/drug effects, Mice, Molecular Sequence Data, Nuclear Proteins/genetics, Trans-Activators/genetics, Transcription Factors/genetics",
author = "{da Silva Xavier}, Gabriela and Gao Sun and Qingwen Qian and Rutter, {Guy A} and Isabelle Leclerc",
note = "Copyright {\textcopyright} 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = nov,
day = "12",
doi = "10.1016/j.bbrc.2010.10.010",
language = "English",
volume = "402",
pages = "252--257",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - ChREBP regulates Pdx-1 and other glucose-sensitive genes in pancreatic β-cells

AU - da Silva Xavier, Gabriela

AU - Sun, Gao

AU - Qian, Qingwen

AU - Rutter, Guy A

AU - Leclerc, Isabelle

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010/11/12

Y1 - 2010/11/12

N2 - Carbohydrate responsive element-binding protein (ChREBP) is a transcription factor whose expression and activity are increased in pancreatic β-cells maintained at elevated glucose concentrations. We show here that ChREBP inactivation in clonal pancreatic MIN6 β-cells results in an increase in Pdx-1 expression at low glucose and to a small, but significant, increase in Ins2, GcK and MafA gene expression at high glucose concentrations. Conversely, adenovirus-mediated over-expression of ChREBP in mouse pancreatic islets results in decreases in Pdx-1, MafA, Ins1, Ins2 and GcK mRNA levels. These data suggest that strategies to reduce ChREBP activity might protect against β-cell dysfunction in type 2 diabetes.

AB - Carbohydrate responsive element-binding protein (ChREBP) is a transcription factor whose expression and activity are increased in pancreatic β-cells maintained at elevated glucose concentrations. We show here that ChREBP inactivation in clonal pancreatic MIN6 β-cells results in an increase in Pdx-1 expression at low glucose and to a small, but significant, increase in Ins2, GcK and MafA gene expression at high glucose concentrations. Conversely, adenovirus-mediated over-expression of ChREBP in mouse pancreatic islets results in decreases in Pdx-1, MafA, Ins1, Ins2 and GcK mRNA levels. These data suggest that strategies to reduce ChREBP activity might protect against β-cell dysfunction in type 2 diabetes.

KW - Animals

KW - Base Sequence

KW - Cell Line, Tumor

KW - Gene Expression Regulation

KW - Gene Silencing

KW - Glucose/metabolism

KW - Homeodomain Proteins/genetics

KW - Insulin-Secreting Cells/drug effects

KW - Mice

KW - Molecular Sequence Data

KW - Nuclear Proteins/genetics

KW - Trans-Activators/genetics

KW - Transcription Factors/genetics

U2 - 10.1016/j.bbrc.2010.10.010

DO - 10.1016/j.bbrc.2010.10.010

M3 - Article

C2 - 20934404

VL - 402

SP - 252

EP - 257

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -