Chitosan primes plant defence mechanisms against Botrytis cinerea, including expression of Avr9/Cf-9 rapidly-elicited genes

Research output: Contribution to journalArticlepeer-review

Authors

  • Daniel De Vega
  • Nicola Holden
  • Peter Hedley
  • Jenny Morris
  • Adrian Newton

Colleges, School and Institutes

External organisations

  • The James Hutton Institute

Abstract

Current crop protection strategies against the fungal pathogen Botrytis cinerea rely on a combination of conventional fungicides and host genetic resistance. However, due to pathogen evolution and legislation in the use of fungicides, these strategies are not sufficient to protect plants against this pathogen. Defence elicitors can stimulate plant defence mechanisms through a phenomenon known as defence priming. Priming results in a faster and/or stronger expression of resistance upon pathogen recognition by the host. This work aims to study defence priming by a commercial formulation of the elicitor chitosan. Treatments with chitosan result in induced resistance (IR) in solanaceous and brassicaceous plants. In tomato plants, enhanced resistance has been linked with priming of callose deposition and accumulation of the plant hormone jasmonic acid (JA). Large-scale transcriptomic analysis revealed that chitosan primes gene expression at early time-points after infection. In addition, two novel tomato genes with a characteristic priming profile were identified, Avr9/Cf-9 rapidly elicited protein 75 (ACRE75) and 180 (ACRE180). Transient and stable over-expression of ACRE75, ACRE180 and their Nicotiana benthamiana homologs, revealed that they are positive regulators of plant resistance against B. cinerea. This provides valuable information in the search for strategies to protect Solanaceae plants against B. cinerea.

Details

Original languageEnglish
Pages (from-to)290-303
Number of pages14
JournalPlant, Cell and Environment
Volume44
Issue number1
Early online date23 Oct 2020
Publication statusPublished - Jan 2021

Keywords

  • Botrytis cinerea (Grey mould), Solanaceae, Solanum lycopersicum (tomato), callose, chitosan, defence priming, induced resistance, transcriptomics

ASJC Scopus subject areas