Chemical methods for N- and O-sulfation of small molecules, amino acids and peptides

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

Sulfation of the amino acid residues of proteins is an emerging post‐translational modification, the functions of which are yet to be fully understood. Current sulfation methods are limited mainly to O ‐tyrosine (sY) which requires negatively charged species around the desired amino acid residue and a specific sulfotransferase enzyme. Alternatively, for solid phase peptide synthesis a de novo protected sY is required. Therefore, synthetic routes are required to go beyond O ‐sulfation. We have developed a novel route to N ‐sulfamation and can dial‐in/out O ‐sulfation (without S ‐sulfurothiolation), mimicking the initiation step of the ping‐pong sulfation mechanism identified in structural biology. This rapid, low temperature and non‐racemizing method is applicable to a range of amines, amides, amino acids, and peptide sequences.

Details

Original languageEnglish
JournalChemBioChem
Early online date6 Nov 2019
Publication statusPublished - 3 Jan 2020

Keywords

  • sulfation, sulfamation, sulfurothiolation, sulfopeptide, modification