Abstract
The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.
Original language | English |
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Pages (from-to) | 2471-2476 |
Number of pages | 6 |
Journal | Biophysical Journal |
Volume | 80 |
Publication status | Published - 1 Jan 2001 |