Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Colleges, School and Institutes

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Details

Original languageEnglish
Title of host publicationOral biology
Subtitle of host publicationmolecular techniques and applications
EditorsGregory J. Seymour, Mary P. Cullinan, Nicholas C. K. Heng
Publication statusPublished - 7 Dec 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1537
ISSN (Print)1064-3745

Keywords

  • Journal Article, Neutrophil extracellular traps , Reactive oxygen species , Fluorescence microscopy , SEM , Chemiluminescence , DNA , Elastase , Myeloperoxidase , Cathepsin G , Immunostaining