Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586.

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Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586. / El-Sayed, AK; Hothersall, Joanne; Cooper, Sian; Stephens, Elton; Simpson, TJ; Thomas, Christopher.

In: Chemistry & Biology, Vol. 10, No. 5, 01.05.2003, p. 419-30.

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@article{264a7518c4804bb2932c09f12800238a,
title = "Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586.",
abstract = "The polyketide antibiotic mupirocin (pseudomonic acid) produced by Pseudomonas fluorescens NCIMB 10586 competitively inhibits bacterial isoleucyl-tRNA synthase and is useful in controlling Staphylococcus aureus, particularly methicillin-resistant Staphylococcus aureus. The 74 kb mupirocin biosynthesis cluster has been sequenced, and putative enzymatic functions of many of the open reading frames (ORFs) have been identified. The mupirocin cluster is a combination of six larger ORFs (mmpA-F), containing several domains resembling the multifunctional proteins of polyketide synthase and fatty acid synthase type I systems, and individual genes (mupA-X and macpA-E), some of which show similarity to type II systems (mupB, mupD, mupG, and mupS). Gene knockout experiments demonstrated the importance of regions in mupirocin production, and complementation of the disrupted gene confirmed that the phenotypes were not due to polar effects. A model for mupirocin biosynthesis is presented based on the sequence and biochemical evidence.",
author = "AK El-Sayed and Joanne Hothersall and Sian Cooper and Elton Stephens and TJ Simpson and Christopher Thomas",
year = "2003",
month = may,
day = "1",
doi = "10.1016/S1074-5521(03)00091-7",
language = "English",
volume = "10",
pages = "419--30",
journal = "Chemistry & Biology",
issn = "1074-5521",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586.

AU - El-Sayed, AK

AU - Hothersall, Joanne

AU - Cooper, Sian

AU - Stephens, Elton

AU - Simpson, TJ

AU - Thomas, Christopher

PY - 2003/5/1

Y1 - 2003/5/1

N2 - The polyketide antibiotic mupirocin (pseudomonic acid) produced by Pseudomonas fluorescens NCIMB 10586 competitively inhibits bacterial isoleucyl-tRNA synthase and is useful in controlling Staphylococcus aureus, particularly methicillin-resistant Staphylococcus aureus. The 74 kb mupirocin biosynthesis cluster has been sequenced, and putative enzymatic functions of many of the open reading frames (ORFs) have been identified. The mupirocin cluster is a combination of six larger ORFs (mmpA-F), containing several domains resembling the multifunctional proteins of polyketide synthase and fatty acid synthase type I systems, and individual genes (mupA-X and macpA-E), some of which show similarity to type II systems (mupB, mupD, mupG, and mupS). Gene knockout experiments demonstrated the importance of regions in mupirocin production, and complementation of the disrupted gene confirmed that the phenotypes were not due to polar effects. A model for mupirocin biosynthesis is presented based on the sequence and biochemical evidence.

AB - The polyketide antibiotic mupirocin (pseudomonic acid) produced by Pseudomonas fluorescens NCIMB 10586 competitively inhibits bacterial isoleucyl-tRNA synthase and is useful in controlling Staphylococcus aureus, particularly methicillin-resistant Staphylococcus aureus. The 74 kb mupirocin biosynthesis cluster has been sequenced, and putative enzymatic functions of many of the open reading frames (ORFs) have been identified. The mupirocin cluster is a combination of six larger ORFs (mmpA-F), containing several domains resembling the multifunctional proteins of polyketide synthase and fatty acid synthase type I systems, and individual genes (mupA-X and macpA-E), some of which show similarity to type II systems (mupB, mupD, mupG, and mupS). Gene knockout experiments demonstrated the importance of regions in mupirocin production, and complementation of the disrupted gene confirmed that the phenotypes were not due to polar effects. A model for mupirocin biosynthesis is presented based on the sequence and biochemical evidence.

U2 - 10.1016/S1074-5521(03)00091-7

DO - 10.1016/S1074-5521(03)00091-7

M3 - Article

C2 - 12770824

VL - 10

SP - 419

EP - 430

JO - Chemistry & Biology

JF - Chemistry & Biology

SN - 1074-5521

IS - 5

ER -