Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation

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Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation. / Müller, E.; Chung, J. T.; Zhang, Z.; Sprauer, A.

In: Journal of Chromatography A, Vol. 1097, No. 1-2, 02.12.2005, p. 116-123.

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@article{fd62e364a98c41f9a1a33ade37b9ca19,
title = "Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation",
abstract = "Chromatographic particles should not possess only desirable surface chemical properties but also mechanical properties. The latter determine the deformation of the particles under hydrodynamic conditions of packed beds and further the pressure drop. Understanding the mechanical properties of chromatographic particles is essential to successful design and operation of such processing equipment. The mechanical properties of single chromatographic particles made of different formulations and with different surface modifications were characterized by a novel micromanipulation technique. The principle of this technique is to compress single particles between two parallel surfaces. The force being imposed on the particles and their deformation under compression are measured simultaneously. The particles range from 30 to 300 μm, depending on their formulation. 10-30 particles from each sample were compressed up to a particle deformation of 70% in order to get statistically representative data. Particles for hydrophobic interaction chromatography were proved more stable than resins for ion exchange or gel filtration. The difference is remarkable in dry state and is less in wet state. Water seems to {"}level out{"} the differences in mechanical stability of resins. The stability seems to be also dependent on the speed of water release out of the resins.",
keywords = "Chromatographic particles, Mechanical stability of modified resins, Micromanipulation method for the determination of mechanical stability, Role of water for the stability of resins",
author = "E. M{\"u}ller and Chung, {J. T.} and Z. Zhang and A. Sprauer",
year = "2005",
month = dec,
day = "2",
doi = "10.1016/j.chroma.2005.08.014",
language = "English",
volume = "1097",
pages = "116--123",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation

AU - Müller, E.

AU - Chung, J. T.

AU - Zhang, Z.

AU - Sprauer, A.

PY - 2005/12/2

Y1 - 2005/12/2

N2 - Chromatographic particles should not possess only desirable surface chemical properties but also mechanical properties. The latter determine the deformation of the particles under hydrodynamic conditions of packed beds and further the pressure drop. Understanding the mechanical properties of chromatographic particles is essential to successful design and operation of such processing equipment. The mechanical properties of single chromatographic particles made of different formulations and with different surface modifications were characterized by a novel micromanipulation technique. The principle of this technique is to compress single particles between two parallel surfaces. The force being imposed on the particles and their deformation under compression are measured simultaneously. The particles range from 30 to 300 μm, depending on their formulation. 10-30 particles from each sample were compressed up to a particle deformation of 70% in order to get statistically representative data. Particles for hydrophobic interaction chromatography were proved more stable than resins for ion exchange or gel filtration. The difference is remarkable in dry state and is less in wet state. Water seems to "level out" the differences in mechanical stability of resins. The stability seems to be also dependent on the speed of water release out of the resins.

AB - Chromatographic particles should not possess only desirable surface chemical properties but also mechanical properties. The latter determine the deformation of the particles under hydrodynamic conditions of packed beds and further the pressure drop. Understanding the mechanical properties of chromatographic particles is essential to successful design and operation of such processing equipment. The mechanical properties of single chromatographic particles made of different formulations and with different surface modifications were characterized by a novel micromanipulation technique. The principle of this technique is to compress single particles between two parallel surfaces. The force being imposed on the particles and their deformation under compression are measured simultaneously. The particles range from 30 to 300 μm, depending on their formulation. 10-30 particles from each sample were compressed up to a particle deformation of 70% in order to get statistically representative data. Particles for hydrophobic interaction chromatography were proved more stable than resins for ion exchange or gel filtration. The difference is remarkable in dry state and is less in wet state. Water seems to "level out" the differences in mechanical stability of resins. The stability seems to be also dependent on the speed of water release out of the resins.

KW - Chromatographic particles

KW - Mechanical stability of modified resins

KW - Micromanipulation method for the determination of mechanical stability

KW - Role of water for the stability of resins

UR - http://www.scopus.com/inward/record.url?scp=27744464370&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2005.08.014

DO - 10.1016/j.chroma.2005.08.014

M3 - Article

C2 - 16298190

AN - SCOPUS:27744464370

VL - 1097

SP - 116

EP - 123

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -