Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro

R Cockeran, H C Steel, A J Theron, T J Mitchell, C Feldman, R Anderson

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P
Original languageEnglish
Pages (from-to)8780-2
Number of pages3
JournalVaccine
Volume29
Issue number48
DOIs
Publication statusPublished - 8 Nov 2011

Bibliographical note

Copyright © 2011 Elsevier Ltd. All rights reserved.

Keywords

  • Bacterial Proteins
  • Calcium
  • Cells, Cultured
  • Humans
  • Leukotriene B4
  • Matrix Metalloproteinase 9
  • Neutrophil Activation
  • Neutrophils
  • Streptolysins
  • Toxoids

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