Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro

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Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro. / Cockeran, R; Steel, H C; Theron, A J; Mitchell, T J; Feldman, C; Anderson, R.

In: Vaccine, Vol. 29, No. 48, 08.11.2011, p. 8780-2.

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Cockeran, R ; Steel, H C ; Theron, A J ; Mitchell, T J ; Feldman, C ; Anderson, R. / Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro. In: Vaccine. 2011 ; Vol. 29, No. 48. pp. 8780-2.

Bibtex

@article{c8d86ec26b8c4eccbf5290144dd00b47,
title = "Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro",
abstract = "The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P",
keywords = "Bacterial Proteins, Calcium, Cells, Cultured, Humans, Leukotriene B4, Matrix Metalloproteinase 9, Neutrophil Activation, Neutrophils, Streptolysins, Toxoids",
author = "R Cockeran and Steel, {H C} and Theron, {A J} and Mitchell, {T J} and C Feldman and R Anderson",
note = "Copyright {\textcopyright} 2011 Elsevier Ltd. All rights reserved.",
year = "2011",
month = nov,
day = "8",
doi = "10.1016/j.vaccine.2011.09.080",
language = "English",
volume = "29",
pages = "8780--2",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "48",

}

RIS

TY - JOUR

T1 - Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro

AU - Cockeran, R

AU - Steel, H C

AU - Theron, A J

AU - Mitchell, T J

AU - Feldman, C

AU - Anderson, R

N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.

PY - 2011/11/8

Y1 - 2011/11/8

N2 - The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P

AB - The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P

KW - Bacterial Proteins

KW - Calcium

KW - Cells, Cultured

KW - Humans

KW - Leukotriene B4

KW - Matrix Metalloproteinase 9

KW - Neutrophil Activation

KW - Neutrophils

KW - Streptolysins

KW - Toxoids

U2 - 10.1016/j.vaccine.2011.09.080

DO - 10.1016/j.vaccine.2011.09.080

M3 - Article

C2 - 21968446

VL - 29

SP - 8780

EP - 8782

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 48

ER -