Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro

Research output: Contribution to journalArticle

Authors

  • R Cockeran
  • H C Steel
  • A J Theron
  • C Feldman
  • R Anderson

Colleges, School and Institutes

Abstract

The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P

Bibliographic note

Copyright © 2011 Elsevier Ltd. All rights reserved.

Details

Original languageEnglish
Pages (from-to)8780-2
Number of pages3
JournalVaccine
Volume29
Issue number48
Publication statusPublished - 8 Nov 2011

Keywords

  • Bacterial Proteins, Calcium, Cells, Cultured, Humans, Leukotriene B4, Matrix Metalloproteinase 9, Neutrophil Activation, Neutrophils, Streptolysins, Toxoids