Characterization of the interactions of the pneumolysoid, Δ6 PLY, with human neutrophils in vitro
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P
Copyright © 2011 Elsevier Ltd. All rights reserved.
|Number of pages||3|
|Publication status||Published - 8 Nov 2011|
- Bacterial Proteins, Calcium, Cells, Cultured, Humans, Leukotriene B4, Matrix Metalloproteinase 9, Neutrophil Activation, Neutrophils, Streptolysins, Toxoids