Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane

Research output: Contribution to journalArticlepeer-review


Colleges, School and Institutes

External organisations

  • Department of Medical Biochemistry and Pharmacology
  • University of Birmingham


1. Rat liver contains a phosphatidylinositol kinase.
2. The enzyme requires phosphatidylinositol, ATP and Mg2+ or Mn2+ for activity.
3. The subcellular distribution of the enzyme does not parallel that of nuclei, mitochondria, lysosomes or endoplasmic reticulum.
4. The kinase and 5'-nucleotidase (a plasma membrane enzyme) are similary distributed in subcellular fractions.
5. The activity of phosphatidylinositol kinase is at least twice as high in purified plasma membrane as in nuclei prepared from the same crude nuclear fraction.
6. The role of the kinase at the cell surface is discussed.


Original languageEnglish
Pages (from-to)649-658
Number of pages10
JournalBBA - Lipids and Lipid Metabolism
Issue number3
Publication statusPublished - 5 Dec 1967

ASJC Scopus subject areas