Characterisation of the dynamic nature of lipids throughout the lifespan of genetically identical female and male Daphnia magna

Research output: Contribution to journalArticlepeer-review

Authors

  • Julia K. Constantinou
  • Martin R. Jones
  • Leda Mirbahai

Colleges, School and Institutes

Abstract

Lipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.

Details

Original languageEnglish
Article number5576
JournalScientific Reports
Volume10
Publication statusPublished - 27 Mar 2020

Keywords

  • Aging/metabolism, Animals, Daphnia/metabolism, Diglycerides/metabolism, Female, Lipid Metabolism/physiology, Longevity/physiology, Male, Phosphatidylcholines/metabolism, Sphingomyelins/metabolism, Triglycerides/metabolism

ASJC Scopus subject areas