TY - JOUR
T1 - Characterisation of the dynamic nature of lipids throughout the lifespan of genetically identical female and male Daphnia magna
AU - Constantinou, Julia K.
AU - Southam, Andrew D.
AU - Kvist, Jouni
AU - Jones, Martin R.
AU - Viant, Mark R.
AU - Mirbahai, Leda
PY - 2020/3/27
Y1 - 2020/3/27
N2 - Lipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.
AB - Lipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.
KW - Aging/metabolism
KW - Animals
KW - Daphnia/metabolism
KW - Diglycerides/metabolism
KW - Female
KW - Lipid Metabolism/physiology
KW - Longevity/physiology
KW - Male
KW - Phosphatidylcholines/metabolism
KW - Sphingomyelins/metabolism
KW - Triglycerides/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85082544653&partnerID=8YFLogxK
U2 - 10.1038/s41598-020-62476-z
DO - 10.1038/s41598-020-62476-z
M3 - Article
C2 - 32221338
AN - SCOPUS:85082544653
SN - 2045-2322
VL - 10
JO - Scientific Reports
JF - Scientific Reports
M1 - 5576
ER -