Characterisation of the dynamic nature of lipids throughout the lifespan of genetically identical female and male Daphnia magna

Research output: Contribution to journalArticle


Colleges, School and Institutes

External organisations

  • Phenome Centre Birmingham
  • Research Program for Molecular Neurology
  • University of Helsinki
  • Swiss Federal Institute of Aquatic Science and Technology
  • Warwick Medical School


Lipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.


Original languageEnglish
Article number5576
JournalScientific Reports
Issue number1
Publication statusPublished - 1 Dec 2020

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