Characterisation of the binding interaction between poly(L-lysine) and DNA using the fluorescamine assay in the preparation of non-viral gene delivery vectors
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A major factor limiting the development of non-viral gene delivery systems is the poor characterisation of polyelectrolyte complexes formed between cationic polymers and DNA. The present study uses the fluorescamine reagent to improve characterisation of poly(L-lysine) (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer by determining the availability of free amino groups. The results show that the fluorescamine reagent can be used to monitor the self-assembly reaction between pLL and DNA and the degree of surface modification of the resultant complexes with a hydrophilic polymer. This experimental approach should enable the preparation of fully defined complexes whose properties can be better related to their biological activity.
|Number of pages||5|
|Publication status||Published - 12 Nov 1999|
- Animals, Cattle, DNA, Dose-Response Relationship, Drug, Ethidium, Fluorescamine, Fluorescent Dyes, Genetic Therapy, Genetic Vectors, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Nucleic Acid Conformation, Polylysine, Polymers, Thymus Gland, Time Factors