Abstract
Polymerase chain reaction studies showed that naphthalene-utilizing bacteria isolated from various localities of Belarus most often contained Nah plasmids of the P-9 incompatibility group and plasmids of indefinite systematics. The conventional incompatibility test and restriction enzyme analysis revealed three new IncP-9 subgroups: xi,eta, and IncP-9-like. In addition to the known nucleotide sequences of nahG and nahAc, two novel nahG variants were revealed by a restriction enzyme analysis of amplification products. An amplified rDNA restriction enzyme analysis (ARDRA) demonstrated that the native hosts of IncP-9 Nah plasmids were fluorescent bacteria of the genus P. seudomonas (P. fluorescens, P. putida, P. aeruginosa, and P. seudomonas sp.) and nonfluorescent bacteria of indefinite systematics.
Original language | English |
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Pages (from-to) | 750-757 |
Number of pages | 8 |
Journal | Molecular Biology |
Volume | 40 |
DOIs | |
Publication status | Published - 1 Oct 2006 |