Characterisation of Nah-plasmids of IncP-9 group from natural strains of pseudomonas (in Russian)

Research output: Contribution to journalArticle

Authors

  • AA Leuchuk
  • IM Bulyha
  • T Izmalkova
  • IA Kosheleva
  • MA Titok

Colleges, School and Institutes

Abstract

Polymerase chain reaction studies showed that naphthalene-utilizing bacteria isolated from various localities of Belarus most often contained Nah plasmids of the P-9 incompatibility group and plasmids of indefinite systematics. The conventional incompatibility test and restriction enzyme analysis revealed three new IncP-9 subgroups: xi,eta, and IncP-9-like. In addition to the known nucleotide sequences of nahG and nahAc, two novel nahG variants were revealed by a restriction enzyme analysis of amplification products. An amplified rDNA restriction enzyme analysis (ARDRA) demonstrated that the native hosts of IncP-9 Nah plasmids were fluorescent bacteria of the genus P. seudomonas (P. fluorescens, P. putida, P. aeruginosa, and P. seudomonas sp.) and nonfluorescent bacteria of indefinite systematics.

Details

Original languageEnglish
Pages (from-to)750-757
Number of pages8
JournalMolecular Biology
Volume40
Publication statusPublished - 1 Oct 2006