Chaperonins facilitate KNOTTED1 cell-to-cell trafficking and stem cell function

Research output: Contribution to journalArticlepeer-review

Authors

  • Xianfeng Morgan Xu
  • Jing Wang
  • Zhenyu Xuan
  • Alexander Goldshmidt
  • Nisha Hariharan
  • Jae Yean Kim
  • David Jackson

Colleges, School and Institutes

External organisations

  • 1] Functional Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA [2].

Abstract

Cell-to-cell communication in plants includes the selective trafficking of transcription factors and other signals through plasmodesmata. The KNOTTED1 (KN1) homeobox (KNOX) family transcription factors, which use this pathway, are essential for stem cell establishment and/or maintenance. Here we show that KN1 trafficking requires the chaperonin complex, which belongs to a group of cytosolic chaperones that fold specific substrate proteins. Genetic and physical interaction data show a functional relevance for chaperonins in KNOX family-dependent stem cell maintenance. Furthermore, tissue-specific complementation assays indicate a mechanistic basis for chaperonin function during the posttranslocational refolding process. Our study shows that chaperonins are essential for the cell-to-cell trafficking of a subset of mobile transcription factors and demonstrates the importance of chaperonin-dependent protein trafficking for plant stem cell function.

Details

Original languageEnglish
Pages (from-to)1141-4
Number of pages4
JournalScience
Volume333
Issue number6046
Publication statusPublished - 26 Aug 2011

Keywords

  • Arabidopsis/cytology, Arabidopsis Proteins/chemistry, Cell Communication, Chaperonins/metabolism, Cytoskeleton/physiology, Homeodomain Proteins/chemistry, Meristem/cytology, Mutation, Plant Leaves/cytology, Plant Proteins/chemistry, Plasmodesmata/metabolism, Protein Folding, Protein Transport, Recombinant Fusion Proteins/chemistry, Zea mays/cytology