Chaperonin Abundance Enhances Bacterial Fitness

C M Santosh Kumar; Kritika Chugh; Anirban Dutta; Vishnuvardhan Mahamkali; Tungadri Bose; Sharmila S Mande; Shekhar C Mande; Peter A Lund

doi:10.3389/fmolb.2021.669996

Chaperonin Abundance Enhances Bacterial Fitness

C M Santosh Kumar, Kritika Chugh, Anirban Dutta, Vishnuvardhan Mahamkali, Tungadri Bose, Sharmila S Mande, Shekhar C Mande, Peter A Lund

Biosciences

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Abstract

The ability of chaperonins to buffer mutations that affect protein folding pathways suggests that their abundance should be evolutionarily advantageous. Here, we investigate the effect of chaperonin overproduction on cellular fitness in Escherichia coli. We demonstrate that chaperonin abundance confers 1) an ability to tolerate higher temperatures, 2) improved cellular fitness, and 3) enhanced folding of metabolic enzymes, which is expected to lead to enhanced energy harvesting potential.

Original language	English
Article number	669996
Journal	Frontiers in Molecular Bioscience
Volume	8
DOIs	https://doi.org/10.3389/fmolb.2021.669996
Publication status	Published - 26 Jul 2021

Bibliographical note

Keywords

GroEL
competitive index
evolution
metabolic flux
metabolism
proteomics

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10.3389/fmolb.2021.669996Licence: Creative Commons: Attribution (CC BY)

fmolb-08-669996Final published version, 1.55 MBLicence: Creative Commons: Attribution (CC BY)

A zebrafish model to study the role of chaperonins in Mycobacterial infection
Lund, P.
Biotechnology & Biological Sciences Research Council
1/05/19 → 31/08/22
Project: Research Councils

Cite this

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title = "Chaperonin Abundance Enhances Bacterial Fitness",

abstract = "The ability of chaperonins to buffer mutations that affect protein folding pathways suggests that their abundance should be evolutionarily advantageous. Here, we investigate the effect of chaperonin overproduction on cellular fitness in Escherichia coli. We demonstrate that chaperonin abundance confers 1) an ability to tolerate higher temperatures, 2) improved cellular fitness, and 3) enhanced folding of metabolic enzymes, which is expected to lead to enhanced energy harvesting potential.",

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AU - Dutta, Anirban

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AU - Bose, Tungadri

AU - Mande, Sharmila S

AU - Mande, Shekhar C

AU - Lund, Peter A

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KW - evolution

KW - metabolic flux

KW - metabolism

KW - proteomics

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JO - Frontiers in Molecular Bioscience

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Chaperonin Abundance Enhances Bacterial Fitness

Abstract

Bibliographical note

Keywords

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A zebrafish model to study the role of chaperonins in Mycobacterial infection

Cite this