CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function
Research output: Contribution to journal › Article › peer-review
Authors
Colleges, School and Institutes
External organisations
- Hubrecht Laboratory KNAW (Netherlands Institute for Developmental Biology)
- Leiden University Medical Center - LUMC
- King's College London
- Utrecht University
Abstract
In recent years, the perception of Z-disc function has changed from a passive anchor for myofilaments that allows transmission of force, to a dynamic multicomplex structure, capable of sensing and transducing extracellular signals. Here, we describe a new Z-disc protein, which we named CHAP (cytoskeletal heart-enriched actin-associated protein), expressed in differentiating heart and skeletal muscle in vitro and in vivo. Interestingly, in addition to its sarcomeric localization, CHAP was also able to translocate to the nucleus. CHAP was associated with filamentous actin in the cytoplasm and the nucleus when expressed ectopically in vitro, but in rat neonatal cardiomyocytes, CHAP disrupted the subcellular localization of a-actinin, another Z-disc protein. More importantly, knockdown of CHAP in zebrafish resulted in aberrant cardiac and skeletal muscle development and function. These findings suggest that CHAP is a critical component of the sarcomere with an important role in muscle development.
Details
Original language | English |
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Pages (from-to) | 1141-1150 |
Number of pages | 10 |
Journal | Journal of Cell Science |
Volume | 123 |
Issue number | 7 |
Publication status | Published - 1 Apr 2010 |
Keywords
- CHAP, Heart development, Muscle, Sarcomere, Synpo2l, Z-disc