Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis

Philip L. Molyneaux; Michael J. Cox; Athol U. Wells; Ho Cheol Kim; Wonjun Ji; William O.C. Cookson; Miriam F. Moffatt; Dong Soon Kim; Toby M. Maher

doi:10.1186/s12931-017-0511-3

Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis

Philip L. Molyneaux, Michael J. Cox, Athol U. Wells, Ho Cheol Kim, Wonjun Ji, William O.C. Cookson, Miriam F. Moffatt, Dong Soon Kim, Toby M. Maher^*

^*Corresponding author for this work

Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.

Original language	English
Article number	29
Journal	Respiratory research
Volume	18
Issue number	1
DOIs	https://doi.org/10.1186/s12931-017-0511-3
Publication status	Published - 1 Feb 2017

Keywords

16S
Acute exacerbation
Bacterial infection
Idiopathic pulmonary fibrosis

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1186/s12931-017-0511-3

Cite this

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title = "Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis",

abstract = "Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.",

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AU - Molyneaux, Philip L.

AU - Cox, Michael J.

AU - Wells, Athol U.

AU - Kim, Ho Cheol

AU - Ji, Wonjun

AU - Cookson, William O.C.

AU - Moffatt, Miriam F.

AU - Kim, Dong Soon

AU - Maher, Toby M.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.

AB - Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.

KW - 16S

KW - Acute exacerbation

KW - Bacterial infection

KW - Idiopathic pulmonary fibrosis

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Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis

Abstract

Keywords

ASJC Scopus subject areas

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