Changes in gene expression and assessment of DNA methylation in primary human hepatocytes and HepG2 cells exposed to the environmental contaminants-Hexabromocyclododecane and 17-beta oestradiol.

SO Aniagu; Timothy Williams; James Chipman

doi:10.1016/j.tox.2008.10.017

Changes in gene expression and assessment of DNA methylation in primary human hepatocytes and HepG2 cells exposed to the environmental contaminants-Hexabromocyclododecane and 17-beta oestradiol.

SO Aniagu, Timothy Williams, James Chipman

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

17 Downloads (Pure)

Abstract

We evaluated the effects of two putative non-genotoxic hepatic carcinogens, hexabromocyclododecane (HBCD) and 17-beta oestradiol (E(2)) on global and CpG promoter DNA methylation in both primary human hepatocytes and hepatocellular carcinoma (HepG2) cells. The mRNA gene expression levels of genes involved particularly in cell cycle were also evaluated and potential correlation with DNA methylation status examined. HBCD at 0.03 and 0.3ng/mL did not produce statistically significant differences in global genomic methylation. However, E(2) (0.1ng/mL) significantly lowered global DNA methylation levels in HepG2 cells by approximately 65% (P

Original language	English
Pages (from-to)	143-51
Number of pages	9
Journal	Toxicology
Volume	256
Issue number	3
DOIs	https://doi.org/10.1016/j.tox.2008.10.017
Publication status	Published - 27 Feb 2009

Keywords

Gene expression
Hepatocytes
17-beta oestradiol
DNA methylation
Hexabromocyclododecane

Access to Document

10.1016/j.tox.2008.10.017

Cite this

@article{e20ce3e0b2c642458a9710c209447ffe,

title = "Changes in gene expression and assessment of DNA methylation in primary human hepatocytes and HepG2 cells exposed to the environmental contaminants-Hexabromocyclododecane and 17-beta oestradiol.",

abstract = "We evaluated the effects of two putative non-genotoxic hepatic carcinogens, hexabromocyclododecane (HBCD) and 17-beta oestradiol (E(2)) on global and CpG promoter DNA methylation in both primary human hepatocytes and hepatocellular carcinoma (HepG2) cells. The mRNA gene expression levels of genes involved particularly in cell cycle were also evaluated and potential correlation with DNA methylation status examined. HBCD at 0.03 and 0.3ng/mL did not produce statistically significant differences in global genomic methylation. However, E(2) (0.1ng/mL) significantly lowered global DNA methylation levels in HepG2 cells by approximately 65% (P",

keywords = "Gene expression, Hepatocytes, 17-beta oestradiol, DNA methylation, Hexabromocyclododecane",

author = "SO Aniagu and Timothy Williams and James Chipman",

year = "2009",

month = feb,

day = "27",

doi = "10.1016/j.tox.2008.10.017",

language = "English",

volume = "256",

pages = "143--51",

journal = "Toxicology",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Changes in gene expression and assessment of DNA methylation in primary human hepatocytes and HepG2 cells exposed to the environmental contaminants-Hexabromocyclododecane and 17-beta oestradiol.

AU - Aniagu, SO

AU - Williams, Timothy

AU - Chipman, James

PY - 2009/2/27

Y1 - 2009/2/27

N2 - We evaluated the effects of two putative non-genotoxic hepatic carcinogens, hexabromocyclododecane (HBCD) and 17-beta oestradiol (E(2)) on global and CpG promoter DNA methylation in both primary human hepatocytes and hepatocellular carcinoma (HepG2) cells. The mRNA gene expression levels of genes involved particularly in cell cycle were also evaluated and potential correlation with DNA methylation status examined. HBCD at 0.03 and 0.3ng/mL did not produce statistically significant differences in global genomic methylation. However, E(2) (0.1ng/mL) significantly lowered global DNA methylation levels in HepG2 cells by approximately 65% (P

AB - We evaluated the effects of two putative non-genotoxic hepatic carcinogens, hexabromocyclododecane (HBCD) and 17-beta oestradiol (E(2)) on global and CpG promoter DNA methylation in both primary human hepatocytes and hepatocellular carcinoma (HepG2) cells. The mRNA gene expression levels of genes involved particularly in cell cycle were also evaluated and potential correlation with DNA methylation status examined. HBCD at 0.03 and 0.3ng/mL did not produce statistically significant differences in global genomic methylation. However, E(2) (0.1ng/mL) significantly lowered global DNA methylation levels in HepG2 cells by approximately 65% (P

KW - Gene expression

KW - Hepatocytes

KW - 17-beta oestradiol

KW - DNA methylation

KW - Hexabromocyclododecane

U2 - 10.1016/j.tox.2008.10.017

DO - 10.1016/j.tox.2008.10.017

M3 - Article

C2 - 19027819

VL - 256

SP - 143

EP - 151

JO - Toxicology

JF - Toxicology

IS - 3

ER -

Changes in gene expression and assessment of DNA methylation in primary human hepatocytes and HepG2 cells exposed to the environmental contaminants-Hexabromocyclododecane and 17-beta oestradiol.

Abstract

Keywords

Access to Document

Fingerprint

Cite this