CD248(+) stromal cells are associated with progressive chronic kidney disease

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CD248(+) stromal cells are associated with progressive chronic kidney disease. / Smith, Stuart; Eardley, KS; Croft, Adam; Nwosu, J; Howie, AJ; Cockwell, Paul; Isacke, CM; Buckley, Christopher; Savage, Caroline.

In: Kidney International, Vol. 80, 13.04.2011, p. 199-207.

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@article{b552de6fca854f5ebff850f825925f1d,
title = "CD248(+) stromal cells are associated with progressive chronic kidney disease",
abstract = "Stromal fibroblasts are the primary cells of the kidney that produce fibrotic matrix. CD248 is a stromal marker expressed on fibroblasts and pericytes within the human kidney. Here, we tested whether CD248 expression in the kidney colocalizes with fibrosis and if it is associated with known determinants of chronic kidney disease (CKD). CD248 expression was located and quantified in situ by immunohistochemistry in kidney biopsies from 93 patients with IgA nephropathy and compared with 22 archived biopsies encompassing normal kidney tissue as control. In normal kidney tissue, CD248 was expressed by resident pericytes, stromal fibroblasts, and was upregulated in human CKD. The expression was linked to known determinants of renal progression. This relationship was maintained in a multivariate analysis with CD248 expression linked to renal survival. CD248 was expressed by a population of α-smooth muscle actin (SMA)(+) myofibroblasts and α-SMA(-) stromal cells but not expressed on CD45(+) leukocytes. Thus, CD248 defines a subset of stromal cells, including but not limited to some myofibroblasts, linked to albuminuria and tubulointerstitial damage during tissue remodeling in CKD.Kidney International advance online publication, 13 April 2011; doi:10.1038/ki.2011.103.",
author = "Stuart Smith and KS Eardley and Adam Croft and J Nwosu and AJ Howie and Paul Cockwell and CM Isacke and Christopher Buckley and Caroline Savage",
year = "2011",
month = apr,
day = "13",
doi = "10.1038/ki.2011.103",
language = "English",
volume = "80",
pages = "199--207",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - CD248(+) stromal cells are associated with progressive chronic kidney disease

AU - Smith, Stuart

AU - Eardley, KS

AU - Croft, Adam

AU - Nwosu, J

AU - Howie, AJ

AU - Cockwell, Paul

AU - Isacke, CM

AU - Buckley, Christopher

AU - Savage, Caroline

PY - 2011/4/13

Y1 - 2011/4/13

N2 - Stromal fibroblasts are the primary cells of the kidney that produce fibrotic matrix. CD248 is a stromal marker expressed on fibroblasts and pericytes within the human kidney. Here, we tested whether CD248 expression in the kidney colocalizes with fibrosis and if it is associated with known determinants of chronic kidney disease (CKD). CD248 expression was located and quantified in situ by immunohistochemistry in kidney biopsies from 93 patients with IgA nephropathy and compared with 22 archived biopsies encompassing normal kidney tissue as control. In normal kidney tissue, CD248 was expressed by resident pericytes, stromal fibroblasts, and was upregulated in human CKD. The expression was linked to known determinants of renal progression. This relationship was maintained in a multivariate analysis with CD248 expression linked to renal survival. CD248 was expressed by a population of α-smooth muscle actin (SMA)(+) myofibroblasts and α-SMA(-) stromal cells but not expressed on CD45(+) leukocytes. Thus, CD248 defines a subset of stromal cells, including but not limited to some myofibroblasts, linked to albuminuria and tubulointerstitial damage during tissue remodeling in CKD.Kidney International advance online publication, 13 April 2011; doi:10.1038/ki.2011.103.

AB - Stromal fibroblasts are the primary cells of the kidney that produce fibrotic matrix. CD248 is a stromal marker expressed on fibroblasts and pericytes within the human kidney. Here, we tested whether CD248 expression in the kidney colocalizes with fibrosis and if it is associated with known determinants of chronic kidney disease (CKD). CD248 expression was located and quantified in situ by immunohistochemistry in kidney biopsies from 93 patients with IgA nephropathy and compared with 22 archived biopsies encompassing normal kidney tissue as control. In normal kidney tissue, CD248 was expressed by resident pericytes, stromal fibroblasts, and was upregulated in human CKD. The expression was linked to known determinants of renal progression. This relationship was maintained in a multivariate analysis with CD248 expression linked to renal survival. CD248 was expressed by a population of α-smooth muscle actin (SMA)(+) myofibroblasts and α-SMA(-) stromal cells but not expressed on CD45(+) leukocytes. Thus, CD248 defines a subset of stromal cells, including but not limited to some myofibroblasts, linked to albuminuria and tubulointerstitial damage during tissue remodeling in CKD.Kidney International advance online publication, 13 April 2011; doi:10.1038/ki.2011.103.

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-79959809001&partnerID=MN8TOARS

U2 - 10.1038/ki.2011.103

DO - 10.1038/ki.2011.103

M3 - Article

C2 - 21490589

VL - 80

SP - 199

EP - 207

JO - Kidney International

JF - Kidney International

SN - 0085-2538

ER -