CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways

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CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways. / Sadej, Rafal; Lu, Xiaohong; Torczyk, Lukasz; Novitskaya, Vera; Lopez-Clavijo, Andrea; Kordek, Radzisław ; Potemski, Piotr; Wakelam, Michael; Romanska, Hanna; Berditchevski, Fedor.

In: Journal of Cell Science, Vol. 131, jcs220640, 29.10.2018.

Research output: Contribution to journalArticlepeer-review

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APA

Sadej, R., Lu, X., Torczyk, L., Novitskaya, V., Lopez-Clavijo, A., Kordek, R., Potemski, P., Wakelam, M., Romanska, H., & Berditchevski, F. (2018). CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways. Journal of Cell Science, 131, [jcs220640]. https://doi.org/10.1242/jcs.220640

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Author

Sadej, Rafal ; Lu, Xiaohong ; Torczyk, Lukasz ; Novitskaya, Vera ; Lopez-Clavijo, Andrea ; Kordek, Radzisław ; Potemski, Piotr ; Wakelam, Michael ; Romanska, Hanna ; Berditchevski, Fedor. / CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways. In: Journal of Cell Science. 2018 ; Vol. 131.

Bibtex

@article{b0c3082821af46808dc3f1ddcd386d95,
title = "CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways",
abstract = "Expression of the tetraspanin CD151 is frequently upregulated in epithelial malignancies and correlates with poor prognosis. Here we report that CD151 is involved in regulation of the expression of fibroblast growth factor receptor 2 (FGFR2). Depletion of CD151 in breast cancer cells resulted in an increased level of FGFR2. Accordingly, an inverse correlation between CD151 and FGFR2 was observed in breast cancer tissues. CD151-dependent regulation of the FGFR2 expression relies on post-transcriptional mechanisms involving HuR/ELAVL1, a multifunctional RNA binding protein, and the assembly of processing bodies (P-bodies). Depletion of CD151 correlated with inhibition of PKC, a well-established downstream target of CD151. Accordingly, the levels of dialcylglycerol species were decreased in CD151-negative cells, and inhibition of PKC resulted in the increased expression of FGFR2. Whilst expression of FGFR2 itself did not correlate with any of the clinicopathological data, the FGFR2-/CD151+ patients are more likely to develop lymph node metastasis. Conversely, FGFR2-/CD151- patients demonstrated better overall survival. These results illustrate functional interdependency between CD151 complexes and FGFR2 and suggest a previously unsuspected role of CD151 in breast tumourigenesis.",
keywords = "CD151, FGFR2, breast cancer, tetraspanin, PKC",
author = "Rafal Sadej and Xiaohong Lu and Lukasz Torczyk and Vera Novitskaya and Andrea Lopez-Clavijo and Radzis{\l}aw Kordek and Piotr Potemski and Michael Wakelam and Hanna Romanska and Fedor Berditchevski",
year = "2018",
month = oct,
day = "29",
doi = "10.1242/jcs.220640",
language = "English",
volume = "131",
journal = "J. Cell Sci",
issn = "0021-9533",
publisher = "The Company of Biologists Ltd.",

}

RIS

TY - JOUR

T1 - CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways

AU - Sadej, Rafal

AU - Lu, Xiaohong

AU - Torczyk, Lukasz

AU - Novitskaya, Vera

AU - Lopez-Clavijo, Andrea

AU - Kordek, Radzisław

AU - Potemski, Piotr

AU - Wakelam, Michael

AU - Romanska, Hanna

AU - Berditchevski, Fedor

PY - 2018/10/29

Y1 - 2018/10/29

N2 - Expression of the tetraspanin CD151 is frequently upregulated in epithelial malignancies and correlates with poor prognosis. Here we report that CD151 is involved in regulation of the expression of fibroblast growth factor receptor 2 (FGFR2). Depletion of CD151 in breast cancer cells resulted in an increased level of FGFR2. Accordingly, an inverse correlation between CD151 and FGFR2 was observed in breast cancer tissues. CD151-dependent regulation of the FGFR2 expression relies on post-transcriptional mechanisms involving HuR/ELAVL1, a multifunctional RNA binding protein, and the assembly of processing bodies (P-bodies). Depletion of CD151 correlated with inhibition of PKC, a well-established downstream target of CD151. Accordingly, the levels of dialcylglycerol species were decreased in CD151-negative cells, and inhibition of PKC resulted in the increased expression of FGFR2. Whilst expression of FGFR2 itself did not correlate with any of the clinicopathological data, the FGFR2-/CD151+ patients are more likely to develop lymph node metastasis. Conversely, FGFR2-/CD151- patients demonstrated better overall survival. These results illustrate functional interdependency between CD151 complexes and FGFR2 and suggest a previously unsuspected role of CD151 in breast tumourigenesis.

AB - Expression of the tetraspanin CD151 is frequently upregulated in epithelial malignancies and correlates with poor prognosis. Here we report that CD151 is involved in regulation of the expression of fibroblast growth factor receptor 2 (FGFR2). Depletion of CD151 in breast cancer cells resulted in an increased level of FGFR2. Accordingly, an inverse correlation between CD151 and FGFR2 was observed in breast cancer tissues. CD151-dependent regulation of the FGFR2 expression relies on post-transcriptional mechanisms involving HuR/ELAVL1, a multifunctional RNA binding protein, and the assembly of processing bodies (P-bodies). Depletion of CD151 correlated with inhibition of PKC, a well-established downstream target of CD151. Accordingly, the levels of dialcylglycerol species were decreased in CD151-negative cells, and inhibition of PKC resulted in the increased expression of FGFR2. Whilst expression of FGFR2 itself did not correlate with any of the clinicopathological data, the FGFR2-/CD151+ patients are more likely to develop lymph node metastasis. Conversely, FGFR2-/CD151- patients demonstrated better overall survival. These results illustrate functional interdependency between CD151 complexes and FGFR2 and suggest a previously unsuspected role of CD151 in breast tumourigenesis.

KW - CD151

KW - FGFR2

KW - breast cancer

KW - tetraspanin

KW - PKC

U2 - 10.1242/jcs.220640

DO - 10.1242/jcs.220640

M3 - Article

VL - 131

JO - J. Cell Sci

JF - J. Cell Sci

SN - 0021-9533

M1 - jcs220640

ER -