cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons

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cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons. / Maiellaro, Isabella; Lohse, Martin J.; Kittel, Robert J.; Calebiro, Davide.

In: Cell Reports, Vol. 17, No. 5, 25.10.2016, p. 1238-1246.

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Maiellaro, Isabella ; Lohse, Martin J. ; Kittel, Robert J. ; Calebiro, Davide. / cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons. In: Cell Reports. 2016 ; Vol. 17, No. 5. pp. 1238-1246.

Bibtex

@article{ce0e3a959ee6464eb9b47007c8908ebd,
title = "cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons",
abstract = "The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons.",
keywords = "active zone, cAMP, dunce, FRET, GPCR, octopamine, PDE, synaptic plasticity",
author = "Isabella Maiellaro and Lohse, {Martin J.} and Kittel, {Robert J.} and Davide Calebiro",
year = "2016",
month = oct,
day = "25",
doi = "10.1016/j.celrep.2016.09.090",
language = "English",
volume = "17",
pages = "1238--1246",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons

AU - Maiellaro, Isabella

AU - Lohse, Martin J.

AU - Kittel, Robert J.

AU - Calebiro, Davide

PY - 2016/10/25

Y1 - 2016/10/25

N2 - The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons.

AB - The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons.

KW - active zone

KW - cAMP

KW - dunce

KW - FRET

KW - GPCR

KW - octopamine

KW - PDE

KW - synaptic plasticity

UR - http://www.scopus.com/inward/record.url?scp=84994709509&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2016.09.090

DO - 10.1016/j.celrep.2016.09.090

M3 - Article

AN - SCOPUS:84994709509

VL - 17

SP - 1238

EP - 1246

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 5

ER -