Abstract
In this study we use carbohydrate probes to search for novel cell surface lectins in the immune system. Many carbohydrate binding proteins are members of the C-type lectin superfamily, whose ligand binding is dependent on calcium. To identify potential new members of this superfamily, fluorescein-conjugated carbohydrate polymers were used to probe for calcium-dependent cell surface binding. This approach offers advantages over the use of monoclonal antibody probes since only carbohydrate binding proteins would be identified. We have identified a cell surface lectin, referred to as thy-lec, detected by the probe fucoidan-fuorescein isothiocyanate. This calcium-dependent lectin has a novel distribution on thymocyte subsets. It is present on the surface of immature CD4+8+ and on large, cycling CD4-8- cells and CD8+4- cells, but not on small, mature phenotype CD8+4- or CD4+8- thymocytes. This lectin is not found on mature T cells or other leukocytes in lymph nodes, spleen or bone marrow. It is proposed that this novel cell surface has a function in the maturation of T cells in the thymus.
Original language | English |
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Pages (from-to) | 77-83 |
Number of pages | 7 |
Journal | European Journal of Immunology |
Volume | 26 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1996 |
Keywords
- Animals
- Calcium
- Carbohydrates
- Cell Differentiation
- Cell Division
- Epitopes
- L-Selectin
- Lectins
- Ligands
- Membrane Proteins
- Mice
- Mice, Inbred C57BL
- T-Lymphocyte Subsets
- Thymus Gland