Ca2+ changes in sympathetic varicosities and Schwann cells in rat mesenteric arteries - relation to noradrenaline release and contraction

Research output: Contribution to journalArticlepeer-review

Authors

  • Thomas Hansen
  • Olga S. Tarasova
  • Makhala M. Khammy
  • Avelino Ferreira
  • James A. Kennard
  • Jørgen Andresen
  • C Staehr
  • Holger Nilsson
  • Christian Aalkjær

Colleges, School and Institutes

External organisations

  • Univ Aarhus
  • Lomonosov Moscow State Univ
  • Aarhus University
  • Department of Pharmacology, University of Oxford, Oxford
  • University of Gothenburg

Abstract

Aim: This study aimed to assess intracellular Ca2+ dynamics in nerve cells and Schwann cells in isolated rat resistance arteries and determine how these dynamics modify noradrenaline release from the nerves and consequent force development.


Methods: Ca2+ in nerves was assessed with confocal imaging, noradrenaline release with amperometry, and artery tone with wire myography. Ca2+ in axons was assessed after loading with Oregon Green 488 BAPTA‐1 dextran. In other experiments, arteries were incubated with Calcium Green‐1‐AM which loads both axons and Schwann cells.


Results: Schwann cells but not axons responded with a Ca2+ increase to ATP. Electrical field stimulation of nerves caused a frequency dependent increase of varicose [Ca2+] ([Ca2+]v). ω‐conotoxin‐GVIA (100 nM) reduced the [Ca2+]v transient to 2 Hz and 16 Hz by 60% and 27%, respectively; in contrast ω‐conotoxin GVIA inhibited more than 80% of the noradrenaline release and force development at 2 and 16 Hz. The KV channel blocker, 4‐aminopyridine (10 μM), increased [Ca2+]v, noradrenaline release, and force development both in the absence and presence of ω‐conotoxin‐GVIA. Yohimbine (1 μM) increased both [Ca2+]v and noradrenaline release but reduced force development. Acetylcholine (10 μM) caused atropine‐sensitive inhibition of [Ca2+]v, noradrenaline release and force. In the presence of ω‐conotoxin‐GVIA, acetylcholine caused a further inhibition of all parameters.

Conclusion: Modification of [Ca2+] in arterial sympathetic axons and Schwann cells was assessed separately. KV3.1 channels may be important regulators of [Ca2+]v, noradrenaline release, and force development. Presynaptic adrenoceptor and muscarinic receptor activation modify transmitter release through modification of [Ca2+]v.

Details

Original languageEnglish
Article numbere13279
JournalActa Physiologica
Volume226
Issue number4
Early online date8 Apr 2019
Publication statusPublished - 31 Aug 2019

Keywords

  • amperometry, confocal imaging, neurotransmission, prejunctional modulation, small arteries, sympathetic

ASJC Scopus subject areas