Both the pre-BCR and the IL-7R alpha are essential for expansion at the pre-BII cell stage in vivo

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Both the pre-BCR and the IL-7R alpha are essential for expansion at the pre-BII cell stage in vivo. / Erlandsson, L; Licence, S; Gaspal, Fabrina; Lane, Peter; Corcoran, AE; Martensson, IL.

In: European Journal of Immunology, Vol. 35, No. 6, 01.06.2005, p. 1969-1976.

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Erlandsson, L ; Licence, S ; Gaspal, Fabrina ; Lane, Peter ; Corcoran, AE ; Martensson, IL. / Both the pre-BCR and the IL-7R alpha are essential for expansion at the pre-BII cell stage in vivo. In: European Journal of Immunology. 2005 ; Vol. 35, No. 6. pp. 1969-1976.

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@article{5841343ca92b4a0383c0b2e8d7c3d7f5,
title = "Both the pre-BCR and the IL-7R alpha are essential for expansion at the pre-BII cell stage in vivo",
abstract = "During B cell development, proliferative expansion takes place after expression of the pre-BCR. At this pre-BII cell stage, the IL-7R alpha is also expressed. Some in vitro studies suggest that pre-BCR-dependent expansion relies on the IL-7R alpha, and others that it does not. It has also been suggested that the pre-BCR mediates down-regulation of the IL-7R alpha. However, the in vivo relationship between the pre-BCR and the IL-7R alpha has not been previously examined. Here, we have investigated this by establishing mice lacking both receptors. Our results show that in the absence of the IL-7R alpha, the pre-BII population is reduced, as previously seen in mice lacking the pre-BCR, demonstrating that the IL-7R alpha is important at this stage. A deficiency in both receptors results in a further reduction of the pre-BII cell population. We conclude that both the IL-7Ra and the pre-BCR are required for optimal pre-BII cell expansion. Furthermore, IL-7R alpha expression levels are normal in pre-BCR-deficient mice, suggesting that the pre-BCR does not mediate its down-regulation. As a consequence of the absence of both receptors, the peripheral B cell pool is severely depleted, resulting in atypical splenic B cell structures and reduced serum Ig levels.",
keywords = "surrogate light chain, B cell development, pre-BCR, pre-B cell proliferation, IL-7R",
author = "L Erlandsson and S Licence and Fabrina Gaspal and Peter Lane and AE Corcoran and IL Martensson",
year = "2005",
month = jun,
day = "1",
doi = "10.1002/eji.200425821",
language = "English",
volume = "35",
pages = "1969--1976",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - Both the pre-BCR and the IL-7R alpha are essential for expansion at the pre-BII cell stage in vivo

AU - Erlandsson, L

AU - Licence, S

AU - Gaspal, Fabrina

AU - Lane, Peter

AU - Corcoran, AE

AU - Martensson, IL

PY - 2005/6/1

Y1 - 2005/6/1

N2 - During B cell development, proliferative expansion takes place after expression of the pre-BCR. At this pre-BII cell stage, the IL-7R alpha is also expressed. Some in vitro studies suggest that pre-BCR-dependent expansion relies on the IL-7R alpha, and others that it does not. It has also been suggested that the pre-BCR mediates down-regulation of the IL-7R alpha. However, the in vivo relationship between the pre-BCR and the IL-7R alpha has not been previously examined. Here, we have investigated this by establishing mice lacking both receptors. Our results show that in the absence of the IL-7R alpha, the pre-BII population is reduced, as previously seen in mice lacking the pre-BCR, demonstrating that the IL-7R alpha is important at this stage. A deficiency in both receptors results in a further reduction of the pre-BII cell population. We conclude that both the IL-7Ra and the pre-BCR are required for optimal pre-BII cell expansion. Furthermore, IL-7R alpha expression levels are normal in pre-BCR-deficient mice, suggesting that the pre-BCR does not mediate its down-regulation. As a consequence of the absence of both receptors, the peripheral B cell pool is severely depleted, resulting in atypical splenic B cell structures and reduced serum Ig levels.

AB - During B cell development, proliferative expansion takes place after expression of the pre-BCR. At this pre-BII cell stage, the IL-7R alpha is also expressed. Some in vitro studies suggest that pre-BCR-dependent expansion relies on the IL-7R alpha, and others that it does not. It has also been suggested that the pre-BCR mediates down-regulation of the IL-7R alpha. However, the in vivo relationship between the pre-BCR and the IL-7R alpha has not been previously examined. Here, we have investigated this by establishing mice lacking both receptors. Our results show that in the absence of the IL-7R alpha, the pre-BII population is reduced, as previously seen in mice lacking the pre-BCR, demonstrating that the IL-7R alpha is important at this stage. A deficiency in both receptors results in a further reduction of the pre-BII cell population. We conclude that both the IL-7Ra and the pre-BCR are required for optimal pre-BII cell expansion. Furthermore, IL-7R alpha expression levels are normal in pre-BCR-deficient mice, suggesting that the pre-BCR does not mediate its down-regulation. As a consequence of the absence of both receptors, the peripheral B cell pool is severely depleted, resulting in atypical splenic B cell structures and reduced serum Ig levels.

KW - surrogate light chain

KW - B cell development

KW - pre-BCR

KW - pre-B cell proliferation

KW - IL-7R

U2 - 10.1002/eji.200425821

DO - 10.1002/eji.200425821

M3 - Article

C2 - 15909309

VL - 35

SP - 1969

EP - 1976

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 6

ER -