BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo

Research output: Contribution to journalArticlepeer-review

Standard

BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo. / Crisan, Mihaela; Solaimani Kartalaei, Parham; Neagu, Alex; Karkanpouna, Sofia; Yamada-Inagawa, Tomoko; Purini, Caterina; Vink, Chris S.; Van Der Linden, Reinier; Van Ijcken, Wilfred; Chuva De Sousa Lopes, Susana M.; Monteiro, Rui; Mummery, Christine; Dzierzak, Elaine.

In: Stem Cell Reports, Vol. 6, No. 3, 08.03.2016, p. 383-395.

Research output: Contribution to journalArticlepeer-review

Harvard

Crisan, M, Solaimani Kartalaei, P, Neagu, A, Karkanpouna, S, Yamada-Inagawa, T, Purini, C, Vink, CS, Van Der Linden, R, Van Ijcken, W, Chuva De Sousa Lopes, SM, Monteiro, R, Mummery, C & Dzierzak, E 2016, 'BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo', Stem Cell Reports, vol. 6, no. 3, pp. 383-395. https://doi.org/10.1016/j.stemcr.2016.01.016

APA

Crisan, M., Solaimani Kartalaei, P., Neagu, A., Karkanpouna, S., Yamada-Inagawa, T., Purini, C., Vink, C. S., Van Der Linden, R., Van Ijcken, W., Chuva De Sousa Lopes, S. M., Monteiro, R., Mummery, C., & Dzierzak, E. (2016). BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo. Stem Cell Reports, 6(3), 383-395. https://doi.org/10.1016/j.stemcr.2016.01.016

Vancouver

Crisan M, Solaimani Kartalaei P, Neagu A, Karkanpouna S, Yamada-Inagawa T, Purini C et al. BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo. Stem Cell Reports. 2016 Mar 8;6(3):383-395. https://doi.org/10.1016/j.stemcr.2016.01.016

Author

Crisan, Mihaela ; Solaimani Kartalaei, Parham ; Neagu, Alex ; Karkanpouna, Sofia ; Yamada-Inagawa, Tomoko ; Purini, Caterina ; Vink, Chris S. ; Van Der Linden, Reinier ; Van Ijcken, Wilfred ; Chuva De Sousa Lopes, Susana M. ; Monteiro, Rui ; Mummery, Christine ; Dzierzak, Elaine. / BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo. In: Stem Cell Reports. 2016 ; Vol. 6, No. 3. pp. 383-395.

Bibtex

@article{d8ffc6eab1024cf38e955d73b01119e2,
title = "BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo",
abstract = "Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.",
keywords = "AGM, BMP, development, hematopoiesis, hematopoietic stem/progenitor cells, Hh, HPSCs, VEGF",
author = "Mihaela Crisan and {Solaimani Kartalaei}, Parham and Alex Neagu and Sofia Karkanpouna and Tomoko Yamada-Inagawa and Caterina Purini and Vink, {Chris S.} and {Van Der Linden}, Reinier and {Van Ijcken}, Wilfred and {Chuva De Sousa Lopes}, {Susana M.} and Rui Monteiro and Christine Mummery and Elaine Dzierzak",
year = "2016",
month = mar,
day = "8",
doi = "10.1016/j.stemcr.2016.01.016",
language = "English",
volume = "6",
pages = "383--395",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo

AU - Crisan, Mihaela

AU - Solaimani Kartalaei, Parham

AU - Neagu, Alex

AU - Karkanpouna, Sofia

AU - Yamada-Inagawa, Tomoko

AU - Purini, Caterina

AU - Vink, Chris S.

AU - Van Der Linden, Reinier

AU - Van Ijcken, Wilfred

AU - Chuva De Sousa Lopes, Susana M.

AU - Monteiro, Rui

AU - Mummery, Christine

AU - Dzierzak, Elaine

PY - 2016/3/8

Y1 - 2016/3/8

N2 - Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.

AB - Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.

KW - AGM

KW - BMP

KW - development

KW - hematopoiesis

KW - hematopoietic stem/progenitor cells

KW - Hh

KW - HPSCs

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=84960803409&partnerID=8YFLogxK

U2 - 10.1016/j.stemcr.2016.01.016

DO - 10.1016/j.stemcr.2016.01.016

M3 - Article

C2 - 26923823

AN - SCOPUS:84960803409

VL - 6

SP - 383

EP - 395

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 3

ER -