BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo

Research output: Contribution to journalArticle

Authors

  • Mihaela Crisan
  • Parham Solaimani Kartalaei
  • Alex Neagu
  • Sofia Karkanpouna
  • Tomoko Yamada-Inagawa
  • Caterina Purini
  • Chris S. Vink
  • Reinier Van Der Linden
  • Wilfred Van Ijcken
  • Susana M. Chuva De Sousa Lopes
  • Christine Mummery
  • Elaine Dzierzak

Colleges, School and Institutes

External organisations

  • Erasmus University Medical Center
  • EDINBURGH UNIVERSITY
  • Leiden University Medical Center - LUMC

Abstract

Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.

Details

Original languageEnglish
Pages (from-to)383-395
Number of pages13
JournalStem Cell Reports
Volume6
Issue number3
Publication statusPublished - 8 Mar 2016

Keywords

  • AGM, BMP, development, hematopoiesis, hematopoietic stem/progenitor cells, Hh, HPSCs, VEGF