Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium

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Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. / Bonkhofer, Florian; Rispoli, Rossella; Pinheiro, Philip; Krecsmarik, Monika; Schneider-Swales, Janina; Tsang, Ingrid Ho Ching; Bruijn, Marella de; Monteiro, Rui; Peterkin, Tessa; Patient, Roger.

In: Nature Communications, Vol. 10, No. 1, 3577, 08.08.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

Bonkhofer, F, Rispoli, R, Pinheiro, P, Krecsmarik, M, Schneider-Swales, J, Tsang, IHC, Bruijn, MD, Monteiro, R, Peterkin, T & Patient, R 2019, 'Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium', Nature Communications, vol. 10, no. 1, 3577. https://doi.org/10.1038/s41467-019-11423-2

APA

Bonkhofer, F., Rispoli, R., Pinheiro, P., Krecsmarik, M., Schneider-Swales, J., Tsang, I. H. C., Bruijn, M. D., Monteiro, R., Peterkin, T., & Patient, R. (2019). Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature Communications, 10(1), [3577]. https://doi.org/10.1038/s41467-019-11423-2

Vancouver

Bonkhofer F, Rispoli R, Pinheiro P, Krecsmarik M, Schneider-Swales J, Tsang IHC et al. Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature Communications. 2019 Aug 8;10(1). 3577. https://doi.org/10.1038/s41467-019-11423-2

Author

Bonkhofer, Florian ; Rispoli, Rossella ; Pinheiro, Philip ; Krecsmarik, Monika ; Schneider-Swales, Janina ; Tsang, Ingrid Ho Ching ; Bruijn, Marella de ; Monteiro, Rui ; Peterkin, Tessa ; Patient, Roger. / Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. In: Nature Communications. 2019 ; Vol. 10, No. 1.

Bibtex

@article{2e0aea4068df4b03b4bfd7fc1ac5bfd6,
title = "Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium",
abstract = "Haematopoietic stem cells are generated from the haemogenic endothelium (HE) located in the floor of the dorsal aorta (DA). Despite being integral to arteries, it is controversial whether HE and arterial endothelium share a common lineage. Here, we present a transgenic zebrafish runx1 reporter line to isolate HE and aortic roof endothelium (ARE)s, excluding non-aortic endothelium. Transcriptomic analysis of these populations identifies Runx1-regulated genes and shows that HE initially expresses arterial markers at similar levels to ARE. Furthermore, runx1 expression depends on prior arterial programming by the Notch ligand dll4. Runx1-/- mutants fail to downregulate arterial genes in the HE, which remains integrated within the DA, suggesting that Runx1 represses the pre-existing arterial programme in HE to allow progression towards the haematopoietic fate. These findings strongly suggest that, in zebrafish, aortic endothelium is a precursor to HE, with potential implications for pluripotent stem cell differentiation protocols for the generation of transplantable HSCs.",
author = "Florian Bonkhofer and Rossella Rispoli and Philip Pinheiro and Monika Krecsmarik and Janina Schneider-Swales and Tsang, {Ingrid Ho Ching} and Bruijn, {Marella de} and Rui Monteiro and Tessa Peterkin and Roger Patient",
year = "2019",
month = aug,
day = "8",
doi = "10.1038/s41467-019-11423-2",
language = "English",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium

AU - Bonkhofer, Florian

AU - Rispoli, Rossella

AU - Pinheiro, Philip

AU - Krecsmarik, Monika

AU - Schneider-Swales, Janina

AU - Tsang, Ingrid Ho Ching

AU - Bruijn, Marella de

AU - Monteiro, Rui

AU - Peterkin, Tessa

AU - Patient, Roger

PY - 2019/8/8

Y1 - 2019/8/8

N2 - Haematopoietic stem cells are generated from the haemogenic endothelium (HE) located in the floor of the dorsal aorta (DA). Despite being integral to arteries, it is controversial whether HE and arterial endothelium share a common lineage. Here, we present a transgenic zebrafish runx1 reporter line to isolate HE and aortic roof endothelium (ARE)s, excluding non-aortic endothelium. Transcriptomic analysis of these populations identifies Runx1-regulated genes and shows that HE initially expresses arterial markers at similar levels to ARE. Furthermore, runx1 expression depends on prior arterial programming by the Notch ligand dll4. Runx1-/- mutants fail to downregulate arterial genes in the HE, which remains integrated within the DA, suggesting that Runx1 represses the pre-existing arterial programme in HE to allow progression towards the haematopoietic fate. These findings strongly suggest that, in zebrafish, aortic endothelium is a precursor to HE, with potential implications for pluripotent stem cell differentiation protocols for the generation of transplantable HSCs.

AB - Haematopoietic stem cells are generated from the haemogenic endothelium (HE) located in the floor of the dorsal aorta (DA). Despite being integral to arteries, it is controversial whether HE and arterial endothelium share a common lineage. Here, we present a transgenic zebrafish runx1 reporter line to isolate HE and aortic roof endothelium (ARE)s, excluding non-aortic endothelium. Transcriptomic analysis of these populations identifies Runx1-regulated genes and shows that HE initially expresses arterial markers at similar levels to ARE. Furthermore, runx1 expression depends on prior arterial programming by the Notch ligand dll4. Runx1-/- mutants fail to downregulate arterial genes in the HE, which remains integrated within the DA, suggesting that Runx1 represses the pre-existing arterial programme in HE to allow progression towards the haematopoietic fate. These findings strongly suggest that, in zebrafish, aortic endothelium is a precursor to HE, with potential implications for pluripotent stem cell differentiation protocols for the generation of transplantable HSCs.

UR - http://www.scopus.com/inward/record.url?scp=85070621659&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-11423-2

DO - 10.1038/s41467-019-11423-2

M3 - Article

C2 - 31395869

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3577

ER -