Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency

EK Halvas; GM Aldrovandi; Peter Balfe; IA Beck; VF Boltz; JM Coffin; LM Frenkel; JD Hazelwood; VA Johnson; M Kearney; A Kovacs; DR Kuritzkes; KJ Metzner; DV Nissley; M Nowicki; S Palmer; R Ziermann; RY Zhao; CL Jennings; J Bremer; D Brambilla; JW Mellors

doi:10.1128/JCM.00449-06

Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency

EK Halvas, GM Aldrovandi, Peter Balfe, IA Beck, VF Boltz, JM Coffin, LM Frenkel, JD Hazelwood, VA Johnson, M Kearney, A Kovacs, DR Kuritzkes, KJ Metzner, DV Nissley, M Nowicki, S Palmer, R Ziermann, RY Zhao, CL Jennings, J BremerD Brambilla, JW Mellors

Immunology and Immunotherapy

Research output: Contribution to journal › Article

102 Citations (Scopus)

Abstract

We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.

Original language	English
Pages (from-to)	2612-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00449-06
Publication status	Published - 1 Jul 2006

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Halvas, EK., Aldrovandi, GM., Balfe, P., Beck, IA., Boltz, VF., Coffin, JM., Frenkel, LM., Hazelwood, JD., Johnson, VA., Kearney, M., Kovacs, A., Kuritzkes, DR., Metzner, KJ., Nissley, DV., Nowicki, M., Palmer, S., Ziermann, R., Zhao, RY., Jennings, CL., ... Mellors, JW. (2006). Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency. Journal of Clinical Microbiology, 44(7), 2612-4. https://doi.org/10.1128/JCM.00449-06

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title = "Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency",

abstract = "We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.",

author = "EK Halvas and GM Aldrovandi and Peter Balfe and IA Beck and VF Boltz and JM Coffin and LM Frenkel and JD Hazelwood and VA Johnson and M Kearney and A Kovacs and DR Kuritzkes and KJ Metzner and DV Nissley and M Nowicki and S Palmer and R Ziermann and RY Zhao and CL Jennings and J Bremer and D Brambilla and JW Mellors",

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Halvas, EK, Aldrovandi, GM, Balfe, P, Beck, IA, Boltz, VF, Coffin, JM, Frenkel, LM, Hazelwood, JD, Johnson, VA, Kearney, M, Kovacs, A, Kuritzkes, DR, Metzner, KJ, Nissley, DV, Nowicki, M, Palmer, S, Ziermann, R, Zhao, RY, Jennings, CL, Bremer, J, Brambilla, D & Mellors, JW 2006, 'Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency', Journal of Clinical Microbiology, vol. 44, no. 7, pp. 2612-4. https://doi.org/10.1128/JCM.00449-06

TY - JOUR

T1 - Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency

AU - Halvas, EK

AU - Aldrovandi, GM

AU - Balfe, Peter

AU - Beck, IA

AU - Boltz, VF

AU - Coffin, JM

AU - Frenkel, LM

AU - Hazelwood, JD

AU - Johnson, VA

AU - Kearney, M

AU - Kovacs, A

AU - Kuritzkes, DR

AU - Metzner, KJ

AU - Nissley, DV

AU - Nowicki, M

AU - Palmer, S

AU - Ziermann, R

AU - Zhao, RY

AU - Jennings, CL

AU - Bremer, J

AU - Brambilla, D

AU - Mellors, JW

PY - 2006/7/1

Y1 - 2006/7/1

N2 - We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.

AB - We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.

U2 - 10.1128/JCM.00449-06

DO - 10.1128/JCM.00449-06

M3 - Article

C2 - 16825395

VL - 44

SP - 2612

EP - 2614

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency

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