Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone

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Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone. / Geng, Hua; Poologasundarampillai, Gowsihan; Todd, Naomi; Devlin, Aine; Moore, Katie L; Golrokhi, Zahra; Gilchrist, James; Jones, Eric; Potter, Richard J; Sutcliffe, Chris.

In: ACS Applied Materials & Interfaces, Vol. 9, No. 25, 05.06.2017, p. 21169–21180.

Research output: Contribution to journalArticle

Harvard

Geng, H, Poologasundarampillai, G, Todd, N, Devlin, A, Moore, KL, Golrokhi, Z, Gilchrist, J, Jones, E, Potter, RJ & Sutcliffe, C 2017, 'Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone', ACS Applied Materials & Interfaces, vol. 9, no. 25, pp. 21169–21180. https://doi.org/10.1021/acsami.7b05150

APA

Vancouver

Author

Geng, Hua ; Poologasundarampillai, Gowsihan ; Todd, Naomi ; Devlin, Aine ; Moore, Katie L ; Golrokhi, Zahra ; Gilchrist, James ; Jones, Eric ; Potter, Richard J ; Sutcliffe, Chris. / Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone. In: ACS Applied Materials & Interfaces. 2017 ; Vol. 9, No. 25. pp. 21169–21180.

Bibtex

@article{f73f6c3b5b2a4c3f8f2d155a9695044a,
title = "Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone",
abstract = "Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.",
author = "Hua Geng and Gowsihan Poologasundarampillai and Naomi Todd and Aine Devlin and Moore, {Katie L} and Zahra Golrokhi and James Gilchrist and Eric Jones and Potter, {Richard J} and Chris Sutcliffe",
year = "2017",
month = "6",
day = "5",
doi = "10.1021/acsami.7b05150",
language = "English",
volume = "9",
pages = "21169–21180",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "25",

}

RIS

TY - JOUR

T1 - Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone

AU - Geng, Hua

AU - Poologasundarampillai, Gowsihan

AU - Todd, Naomi

AU - Devlin, Aine

AU - Moore, Katie L

AU - Golrokhi, Zahra

AU - Gilchrist, James

AU - Jones, Eric

AU - Potter, Richard J

AU - Sutcliffe, Chris

PY - 2017/6/5

Y1 - 2017/6/5

N2 - Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.

AB - Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.

U2 - 10.1021/acsami.7b05150

DO - 10.1021/acsami.7b05150

M3 - Article

VL - 9

SP - 21169

EP - 21180

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 25

ER -