Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390

Research output: Contribution to journalArticle

Authors

  • Annabel C. Murphy
  • Shu Shan Gao
  • Li Chen Han
  • Simon Carobene
  • Daisuke Fukuda
  • Zhongshu Song
  • Russell J. Cox
  • John Crosby
  • Matthew P. Crump
  • Christopher M. Thomas
  • Christine L. Willis
  • Thomas J. Simpson

Colleges, School and Institutes

External organisations

  • Japan Agency for Marine-Earth Science and Technology
  • University of Cambridge
  • BRISTOL UNIVERSITY
  • Department of Biochemistry

Abstract

The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.

Details

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalChemical Science
Volume5
Issue number1
Publication statusPublished - Jan 2014

ASJC Scopus subject areas