Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Research output: Contribution to journalArticle

Authors

  • Shu Shan Gao
  • Ji'En Wu
  • Annabel C. Murphy
  • Zhongshu Song
  • Elton R. Stephens
  • Christopher M. Thomas
  • Matthew P. Crump
  • Russell J. Cox
  • Thomas J. Simpson
  • Christine L. Willis

Colleges, School and Institutes

External organisations

  • University of Cambridge
  • BRISTOL UNIVERSITY
  • Department of Biochemistry
  • Institute of Molecular and Cell Biology, A-Star, Singapore

Abstract

Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Details

Original languageEnglish
Pages (from-to)5501-5507
Number of pages7
JournalJournal of the American Chemical Society
Volume136
Issue number14
Publication statusPublished - 9 Apr 2014