Biosensors for detection of mercury in contaminated soils

Research output: Contribution to journalArticle

Authors

  • I Bontidean
  • A Mortari
  • S Leth
  • U Karlson
  • MM Larsen
  • J Vrangonsveld

Colleges, School and Institutes

Abstract

Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities.

Details

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalEnvironmental Pollution
Volume131
Publication statusPublished - 1 Jan 2004

Keywords

  • capacitive biosensor, plant sensor, whole cell, soil, mercury