Bio-inspired mini-eggs with pH-responsive membrane for enzyme immobilization

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Bio-inspired mini-eggs with pH-responsive membrane for enzyme immobilization. / Mei, Li; Xie, Rui; Yang, Chao; Ju, Xiao Jie; Wang, Ji Yun; Zhang, Zhibing; Chu, Liang Yin.

In: Journal of Membrane Science, Vol. 429, 05.02.2013, p. 313-322.

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Mei, Li ; Xie, Rui ; Yang, Chao ; Ju, Xiao Jie ; Wang, Ji Yun ; Zhang, Zhibing ; Chu, Liang Yin. / Bio-inspired mini-eggs with pH-responsive membrane for enzyme immobilization. In: Journal of Membrane Science. 2013 ; Vol. 429. pp. 313-322.

Bibtex

@article{c6a4089101d543b2b04a9ffca7d7b145,
title = "Bio-inspired mini-eggs with pH-responsive membrane for enzyme immobilization",
abstract = "A novel family of bio-inspired mini-eggs with a pH-responsive membrane for enzyme immobilization has been designed and prepared by a simple and mild method combining co-extrusion minifluidics, adsorption and biosilicification. The membrane of the proposed mini-eggs is composed of a Ca-alginate/protamine composite layer encapsulated by a silica surface layer, in which the former provides the pH-responsive switching function driven by electrostatic interactions between Ca-alginate networks and protamine molecules, and the latter offers protection for the former to guarantee the stability of pH-responsive performance. When the ambient pH value is lower than a certain threshold (called critical pH value), the enzymatic reaction in the mini-eggs is inactive due to the low diffusional permeability of substrates and products across the membrane; however, when the pH value is higher than the threshold, the enzymatic reaction in the mini-eggs becomes active due to the high diffusional permeability across the membrane. The pH-responsive switching action of the mini-egg membrane is rapid, reversible and reproducible. The proposed mini-eggs provide a new mode of {"}smart{"} or {"}intelligent{"} controllable mini-systems for pH-responsive enzymatic reactions, which is highly attractive for enzyme catalytic reaction, cell and tissue culture, sensors, etc.",
keywords = "Capsule membranes, Composite membranes, Enzyme immobilization, Mini-egg-like capsules, PH-responsive membranes",
author = "Li Mei and Rui Xie and Chao Yang and Ju, {Xiao Jie} and Wang, {Ji Yun} and Zhibing Zhang and Chu, {Liang Yin}",
year = "2013",
month = feb,
day = "5",
doi = "10.1016/j.memsci.2012.11.067",
language = "English",
volume = "429",
pages = "313--322",
journal = "Journal of Membrane Science",
issn = "0376-7388",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Bio-inspired mini-eggs with pH-responsive membrane for enzyme immobilization

AU - Mei, Li

AU - Xie, Rui

AU - Yang, Chao

AU - Ju, Xiao Jie

AU - Wang, Ji Yun

AU - Zhang, Zhibing

AU - Chu, Liang Yin

PY - 2013/2/5

Y1 - 2013/2/5

N2 - A novel family of bio-inspired mini-eggs with a pH-responsive membrane for enzyme immobilization has been designed and prepared by a simple and mild method combining co-extrusion minifluidics, adsorption and biosilicification. The membrane of the proposed mini-eggs is composed of a Ca-alginate/protamine composite layer encapsulated by a silica surface layer, in which the former provides the pH-responsive switching function driven by electrostatic interactions between Ca-alginate networks and protamine molecules, and the latter offers protection for the former to guarantee the stability of pH-responsive performance. When the ambient pH value is lower than a certain threshold (called critical pH value), the enzymatic reaction in the mini-eggs is inactive due to the low diffusional permeability of substrates and products across the membrane; however, when the pH value is higher than the threshold, the enzymatic reaction in the mini-eggs becomes active due to the high diffusional permeability across the membrane. The pH-responsive switching action of the mini-egg membrane is rapid, reversible and reproducible. The proposed mini-eggs provide a new mode of "smart" or "intelligent" controllable mini-systems for pH-responsive enzymatic reactions, which is highly attractive for enzyme catalytic reaction, cell and tissue culture, sensors, etc.

AB - A novel family of bio-inspired mini-eggs with a pH-responsive membrane for enzyme immobilization has been designed and prepared by a simple and mild method combining co-extrusion minifluidics, adsorption and biosilicification. The membrane of the proposed mini-eggs is composed of a Ca-alginate/protamine composite layer encapsulated by a silica surface layer, in which the former provides the pH-responsive switching function driven by electrostatic interactions between Ca-alginate networks and protamine molecules, and the latter offers protection for the former to guarantee the stability of pH-responsive performance. When the ambient pH value is lower than a certain threshold (called critical pH value), the enzymatic reaction in the mini-eggs is inactive due to the low diffusional permeability of substrates and products across the membrane; however, when the pH value is higher than the threshold, the enzymatic reaction in the mini-eggs becomes active due to the high diffusional permeability across the membrane. The pH-responsive switching action of the mini-egg membrane is rapid, reversible and reproducible. The proposed mini-eggs provide a new mode of "smart" or "intelligent" controllable mini-systems for pH-responsive enzymatic reactions, which is highly attractive for enzyme catalytic reaction, cell and tissue culture, sensors, etc.

KW - Capsule membranes

KW - Composite membranes

KW - Enzyme immobilization

KW - Mini-egg-like capsules

KW - PH-responsive membranes

UR - http://www.scopus.com/inward/record.url?scp=84871553785&partnerID=8YFLogxK

U2 - 10.1016/j.memsci.2012.11.067

DO - 10.1016/j.memsci.2012.11.067

M3 - Article

AN - SCOPUS:84871553785

VL - 429

SP - 313

EP - 322

JO - Journal of Membrane Science

JF - Journal of Membrane Science

SN - 0376-7388

ER -