TY - JOUR
T1 - Biochemical and behavioural responses of the marine polychaete Hediste diversicolor to cadmium sulfide quantum dots (CdS QDs)
T2 - waterborne and dietary exposure
AU - Buffet, Pierre-Emmanuel
AU - Poirier, Laurence
AU - Zalouk-Vergnoux, Aurore
AU - Lopes, Christelle
AU - Amiard, Jean-Claude
AU - Gaudin, Pierre
AU - Risso-de Faverney, Christine
AU - Guibbolini, Marielle
AU - Gilliland, Douglas
AU - Perrein-Ettajani, Hanane
AU - Valsami-Jones, Eugenia
AU - Mouneyrac, Catherine
N1 - Copyright © 2014 Elsevier Ltd. All rights reserved.
PY - 2014/4
Y1 - 2014/4
N2 - Cadmium sulfide (CdS) quantum dots are widely used in medical imaging. The aim of this study was to examine toxicity effects of CdS engineered nanoparticles (CdS NPs) compared to soluble Cd, on marine ragworms (Hediste diversicolor) exposed for 14 d to these contaminants (10 μg Cd L(-1)) in seawater or via their food (contaminated worm tissue). In our experimental media, Dynamic Light Scattering studies showed that the majority of CdS remained in the nanoscale (1-10 nm) with the exception of few aggregates (100-300 nm). Labile Cd fractions released from CdS NPs were estimated by diffusive gradient in thin films, showing that about 50% of CdS NPs remained in nanoparticulate form. Ragworms accumulated Cd in both soluble Cd and CdS NPs in waterborne exposures only. Greater significant changes of biochemical responses were observed in worms exposed to CdS NPs in seawater compared to contaminated food. Catalase and glutathione-S-transferase activities were the most sensitive biochemical biomarkers responding to both Cd treatments for waterborne exposure. Inductions of CAT were higher in diet-exposed worms to Cd as NPs vs soluble form suggesting a specific "nano" effect. Caspase activities increased in worms exposed to soluble Cd and Cd NPs for the two routes of exposure compared to controls. Defences, may be insufficient to prevent reactive oxygen species generation and the associated apoptosis. Behaviour of invertebrates inside sediment showed impairments of body movements in worms exposed to CdS NPs. This study points out oxidative processes as the main consequences of exposure to Cd based NPs in worms.
AB - Cadmium sulfide (CdS) quantum dots are widely used in medical imaging. The aim of this study was to examine toxicity effects of CdS engineered nanoparticles (CdS NPs) compared to soluble Cd, on marine ragworms (Hediste diversicolor) exposed for 14 d to these contaminants (10 μg Cd L(-1)) in seawater or via their food (contaminated worm tissue). In our experimental media, Dynamic Light Scattering studies showed that the majority of CdS remained in the nanoscale (1-10 nm) with the exception of few aggregates (100-300 nm). Labile Cd fractions released from CdS NPs were estimated by diffusive gradient in thin films, showing that about 50% of CdS NPs remained in nanoparticulate form. Ragworms accumulated Cd in both soluble Cd and CdS NPs in waterborne exposures only. Greater significant changes of biochemical responses were observed in worms exposed to CdS NPs in seawater compared to contaminated food. Catalase and glutathione-S-transferase activities were the most sensitive biochemical biomarkers responding to both Cd treatments for waterborne exposure. Inductions of CAT were higher in diet-exposed worms to Cd as NPs vs soluble form suggesting a specific "nano" effect. Caspase activities increased in worms exposed to soluble Cd and Cd NPs for the two routes of exposure compared to controls. Defences, may be insufficient to prevent reactive oxygen species generation and the associated apoptosis. Behaviour of invertebrates inside sediment showed impairments of body movements in worms exposed to CdS NPs. This study points out oxidative processes as the main consequences of exposure to Cd based NPs in worms.
KW - Animals
KW - Behavior, Animal
KW - Biological Markers
KW - Cadmium Compounds
KW - Diet
KW - Polychaeta
KW - Quantum Dots
KW - Seawater
KW - Solubility
KW - Sulfides
U2 - 10.1016/j.chemosphere.2013.12.069
DO - 10.1016/j.chemosphere.2013.12.069
M3 - Article
C2 - 24480429
SN - 0045-6535
VL - 100
SP - 63
EP - 70
JO - Chemosphere
JF - Chemosphere
ER -