Binding of the periplakin linker requires vimentin acidic residues D176 and E187

Research output: Contribution to journalArticle

Authors

  • Catharine Trieber
  • Penelope Rodriguez-Zamora
  • Caezar Al-Jassa
  • Tzu-Han Huang
  • Claudia Fogl
  • Pooja Sridhar
  • Jitendra Kumar
  • Michael Overduin

External organisations

  • University of Alberta
  • Universidad Nacional Autónoma de México
  • The Binding Site, Edgbaston, Birmingham, United Kingdom.
  • University of Alberta

Abstract

Plakin proteins form connections that link the cell membrane to the intermediate filament cytoskeleton. Their interactions are mediated by a highly conserved linker domain through an unresolved mechanism. Here analysis of the human periplakin linker domain structure reveals a bi-lobed module transected by an electropositive groove. Key basic residues within the periplakin groove are vital for co-localization with vimentin in human cells and compromise direct binding which also requires acidic residues D176 and E187 in vimentin. We propose a model whereby basic periplakin linker domain residues recognize acidic vimentin side chains and form a complementary binding groove. The model is shared amongst diverse linker domains and can be used to investigate the effects of pathogenic mutations in the desmoplakin linker associated with arrhythmogenic right ventricular cardiomyopathy. Linker modules either act solely or collaborate with adjacent plakin repeat domains to create strong and adaptable tethering within epithelia and cardiac muscle.

Details

Original languageEnglish
Article number83
Number of pages13
JournalCommunications Biology
Volume3
Issue number1
Publication statusPublished - 21 Feb 2020