Abstract
We have investigated the reversibility of biochemical and physiological changes that occur upon suspension of ejaculated human spermatozoa during in vitro capacitation. Cells were swum up in a simple HEPES-based saline [lacking bicarbonate and bovine serum albumin (BSA)], then resuspended either in supplemented Earle's balanced salt solution (sEBSS) (25 mM bicarbonate) with 0.3% BSA (for in vitro capacitation) or in medium-lacking bicarbonate and/or BSA. Progesterone-induced acrosome reaction (AR) developed during in vitro capacitation (6 h). A progesterone-induced [Ca2+](i) signal was detectable in cells maintained in the simple HEPES-based saline, but upon transfer to sEBSS, the response increased three- to four-fold, saturating within
Original language | English |
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Pages (from-to) | 683-691 |
Number of pages | 9 |
Journal | Molecular Human Reproduction |
Volume | 11 |
Issue number | 9 |
DOIs | |
Publication status | Published - 11 Oct 2005 |
Keywords
- calcium
- sperm
- capacitation
- phosphorylation
- cAMP